Tumor-associated macrophages (TAMs) have essential roles in
tumor progression and
metastasis.
Tumor cells recruit myeloid progenitors and monocytes to the
tumor site, where they differentiate into TAMs; however, this process is not well studied in humans. Here we show that human CD7, a T-cell and
NK cell receptor, is highly expressed by monocytes and macrophages. Expression of CD7 decreases in
M-CSF-differentiated macrophages and in
melanoma-
conditioned medium-induced macrophages (MCMI/Mφ) in comparison to monocytes. A
ligand for CD7, SECTM1 (secreted and transmembrane
protein 1), is highly expressed in many
tumors, including
melanoma cells. We show that SECTM1 binds to CD7 and significantly increases monocyte migration by activation of the PI3K (phosphatidylinositol 3'-kinase) pathway. In human
melanoma tissues,
tumor-infiltrating macrophages expressing CD7 are present. These
melanomas, with CD7-positive inflammatory cell infiltrations, frequently highly express SECTM1, including an N-terminal, soluble form, which can be detected in the sera of metastatic
melanoma patients but not in normal sera. Taken together, our data demonstrate that CD7 is present on monocytes and
tumor macrophages and that its
ligand, SECTM1, is frequently expressed in corresponding
melanoma tissues, possibly acting as a
chemoattractant for monocytes to modulate the
melanoma microenvironment.