[Effect of PC cell-derived growth factor RNA interference on biological behavior of esophageal squamous carcinoma cells].

Abstract

OBJECTIVE:

To investigate the effect of PC cell-derived growth factor (PCDGF) RNA interference on esophageal squamous carcinoma cells Eca-109 in vitro.

METHODS:

The PCDGF-shRNA expression vector was transfected into the Eca-109 cells by liposome. After transfection, the mRNA and protein expressions of PCDGF were detected by RT-PCR and Western-blot respectively. Cell Counting Kit-8 (CCK-8) assay and Boyden chamber method were performed to measure the cell proliferation and invasion ability respectively.

RESULTS:

The expression levels of PCDGF mRNA and protein were both decreased in Eca-109 cells transfected with PCDGF-shRNA expression vector (transfection group). Twenty-four, 48 and 72 h after transfection, the cells proliferation in the transfection group was inhibited, and the inhibition rate was 20.4%, 21.1% and 20.9% respectively. The cell proliferation activity in the transfection group was significantly lower than that in the non-transfection group, liposome group and negative vector group (all P<0.05). The number of cell migration in the non-transfection group,negative vector group, liposome group and transfection group was 118.8±12.0, 100.8±9.0, 114.3±4.7, and 53.5±16.3 respectively. The differences were statistically significant between the transfection group and the other 3 groups (all P<0.05).

CONCLUSIONS:

PCDGF RNA interference can inhibit the proliferation and invasion abilities of esophageal squamous carcinoma cells in vitro. PCDGF gene may be the new target of gene therapy.

• Authors: Qing-feng Zheng, Shuo-yan Liu, Hai-yan Wang, Feng Wang, Zhen Wang, Xiao-feng Chen, Jian-jian Wang, Min-gang Ying, Xiong-wei Zheng, Xian-dong Lin, Zhi-feng Zhou, Fu-sheng Gong, Yun-qing Xie
• Journal: Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery (Zhonghua Wei Chang Wai Ke Za Zhi) Vol. 16 Issue 9 Pg. 874-7 (Sep 2013) ISSN: 1671-0274 [Print] China
• PMID: 24061998 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• GRN protein, human
• Intercellular Signaling Peptides and Proteins
• Progranulins
• RNA, Small Interfering

Topics

• Carcinoma, Squamous Cell (metabolism, pathology)
• Cell Line, Tumor
• Cell Proliferation
• Esophageal Neoplasms (metabolism, pathology)
• Esophageal Squamous Cell Carcinoma
• Genetic Vectors
• Humans
• Intercellular Signaling Peptides and Proteins (genetics, metabolism)
• Progranulins
• RNA Interference
• RNA, Small Interfering (genetics)
• Transfection