Abstract |
African horse sickness virus (AHSV) belongs to the genus Orbivirus. We have now engineered naked DNAs and recombinant modified vaccinia virus Ankara (rMVA) expressing VP2 and NS1 proteins from AHSV-4. IFNAR((-/-)) mice inoculated with DNA/rMVA-VP2,-NS1 from AHSV-4 in an heterologous prime-boost vaccination strategy generated significant levels of neutralizing antibodies specific of AHSV-4. In addition, vaccination stimulated specific T cell responses against the virus. The vaccine elicited partial protection against an homologous AHSV-4 infection and induced cross-protection against the heterologous AHSV-9. Similarly, IFNAR((-/-)) mice vaccinated with an homologous prime-boost strategy with rMVA-VP2-NS1 from AHSV-4 developed neutralizing antibodies and protective immunity against AHSV-4. Furthermore, the levels of immunity were very high since none of vaccinated animals presented viraemia when they were challenged against the homologous AHSV-4 and very low levels when they were challenged against the heterologous virus AHSV-9. These data suggest that the immunization with rMVA/rMVA was more efficient in protection against a virulent challenge with AHSV-4 and both strategies, DNA/rMVA and rMVA/rMVA, protected against the infection with AHSV-9. The inclusion of the protein NS1 in the vaccine formulations targeting AHSV generates promising multiserotype vaccines.
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Authors | Francisco de la Poza, Eva Calvo-Pinilla, Elena López-Gil, Alejandro Marín-López, Francisco Mateos, Javier Castillo-Olivares, Gema Lorenzo, Javier Ortego |
Journal | PloS one
(PLoS One)
Vol. 8
Issue 7
Pg. e70197
( 2013)
ISSN: 1932-6203 [Electronic] United States |
PMID | 23894615
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Ifnar1 protein, mouse
- NS1 protein, African horse sickness virus
- Vaccines, Synthetic
- Viral Nonstructural Proteins
- Viral Vaccines
- Receptor, Interferon alpha-beta
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Topics |
- African Horse Sickness
(immunology, prevention & control)
- African Horse Sickness Virus
(classification, immunology)
- Animals
- Cell Line
- Chlorocebus aethiops
- Disease Models, Animal
- Horses
- Male
- Mice
- Mice, 129 Strain
- Receptor, Interferon alpha-beta
(genetics)
- Vaccines, Synthetic
(genetics, immunology)
- Vero Cells
- Viral Nonstructural Proteins
(genetics, immunology)
- Viral Vaccines
(immunology)
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