Neuronal damage using fluoro-Jade B histofluorescence and gliosis in the gerbil septum submitted to various durations of cerebral ischemia.

Abstract

The extent of neuronal damage/death in some brain regions is highly correlated to duration time of transient ischemia. In the present study, we carried out neuronal degeneration/death and glial changes in the septum 4 days after 5, 10, 15, and 20 min of transient cerebral ischemia using gerbils. To examine neuronal damage, Fluoro-Jade B (F-J B, a marker for neuronal degeneration) histofluorescence staining was used. F-J B positive ((+)) cells were detected in the septo-hippocampal nucleus (SHN) of the septum only in the 20 min ischemia-group; the mean number of F-J B(+) neurons was 14.9 ± 2.5/400 μm(2) in a section. Gliosis of astrocytes and microglia was examined using anti-glial fibrillary acidic protein (GFAP) and anti-ionized calcium-binding adapter molecule 1 (Iba-1), respectively. In all the ischemia-groups, GFAP- and Iba-1-immunoreactive astrocytes and microglia, respectively, were increased in number, and apparently tended to be increased in their immunoreactivity. Especially, in the 20 min ischemia-group, the number and immunoreactivity of Iba-immunoreactive microglia was highest and strongest in the ischemic SHN 4 days after ischemia-reperfusion. In brief, our findings showed that neuronal damage/death in the SHN occurred and gliosis was apparently increased in the 20 min ischemia-group at 4 days after ischemia-reperfusion.

• Authors: Chan Woo Park, Jae-Chul Lee, Ji Hyeon Ahn, Dae Hwan Lee, Geum-Sil Cho, Bing Chun Yan, Joon Ha Park, In Hye Kim, Hui Young Lee, Moo-Ho Won, Jun Hwi Cho
• Journal: Cellular and molecular neurobiology (Cell Mol Neurobiol) Vol. 33 Issue 7 Pg. 991-1001 (Oct 2013) ISSN: 1573-6830 [Electronic] United States
• PMID: 23893372 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Benzoxazines
• Fluoresceins
• Glial Fibrillary Acidic Protein
• Microfilament Proteins
• Nerve Tissue Proteins
• fluoro jade
• cresyl violet

Topics

• Animals
• Astrocytes (metabolism, pathology)
• Benzoxazines
• Brain Ischemia (complications, metabolism, pathology)
• Fluoresceins (metabolism)
• Fluorescent Antibody Technique
• Gerbillinae (metabolism)
• Glial Fibrillary Acidic Protein (metabolism)
• Gliosis (complications, metabolism, pathology)
• Male
• Microfilament Proteins (metabolism)
• Microglia (metabolism, pathology)
• Nerve Tissue Proteins (metabolism)
• Neurons (metabolism, pathology)
• Septum of Brain (metabolism, pathology)
• Staining and Labeling