Mouse mannose-binding lectin-A and ficolin-A inhibit lipopolysaccharide-mediated pro-inflammatory responses on mast cells.

Abstract

It is unknown how soluble pattern-recognition receptors in blood, such as mannose-binding lectin (MBL) and ficolins, modulate mast cell-mediated inflammatory responses. We investigate how mouse MBL-A or ficolin-A regulate mouse bone marrow-derived mast cells (mBMMCs)-derived inflammatory response against bacterial lipopolysaccharide (LPS) stimulation. LPS-mediated pro-inflammatory cytokine productions on mBMMCs obtained from Toll-like receptor4 (TLR4)-deficient mice, TLR2-defficient mice, and their wildtype, were specifically attenuated by the addition of either mouse MBL-A or ficolin-A in a dose-dependent manner. However, the inhibitory effects by mouse MBL-A or ficolin-A were restored by the addition of mannose or N-acetylglucosamine, respectively. These results suggest that mouse MBL-A and ficolin-A bind to LPS via its carbohydrate-recognition domain and fibrinogen-like domain, respectively, whereby cytokine production by LPS-mediated TLR4 in mBMMCs appears to be down-regulated, indicating that mouse MBL and ficolin may have an inhibitory function toward mouse TLR4-mediated excessive inflammation on the mast cells.

• Authors: Ying Jie Ma, Hee Jung Kang, Ji Yeon Kim, Peter Garred, Myung-Shik Lee, Bok Luel Lee
• Journal: BMB reports (BMB Rep) Vol. 46 Issue 7 Pg. 376-81 (Jul 2013) ISSN: 1976-670X [Electronic] Korea (South)
• PMID: 23884105 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cytokines
• Inflammation Mediators
• Interleukin-6
• Lectins
• Lipopolysaccharides
• Mannose-Binding Lectin
• Toll-Like Receptor 4
• Mannose
• Acetylglucosamine

Topics

• Acetylglucosamine (pharmacology)
• Animals
• Bone Marrow Cells (cytology)
• Cells, Cultured
• Cytokines (metabolism)
• Inflammation Mediators (metabolism)
• Interleukin-6 (metabolism)
• Lectins (metabolism, pharmacology)
• Lipopolysaccharides (metabolism, toxicity)
• Mannose (pharmacology)
• Mannose-Binding Lectin (metabolism, pharmacology)
• Mast Cells (drug effects, immunology, metabolism)
• Mice
• Mice, Inbred C57BL
• Mice, Knockout
• Protein Binding
• Toll-Like Receptor 4 (deficiency, genetics, metabolism)
• Ficolins