Abstract | PURPOSE: METHODS: Male Wistar rats were randomly divided into sham, shock, shock+ligation ( shock plus mesenteric lymph duct ligation (MLDL)), shock+drainage ( shock plus PSML drainage) groups. After shock ( hypotension 40 mm Hg) for three hours or corresponding times, the superior mesenteric artery (SMA) was taken out for detecting the PKG and phospho PKG (p-PKG) contents, and the vascular rings of SMA were prepared for assaying the calcium sensitivity using an isolated organ perfusion system. RESULTS: The PKG and p-PKG contents of SMA in shock group were significantly increased than that of sham group, and MLDL or PSML drainage reducing the levels of PKG and p-PKG. Meanwhile, the vascular calcium sensitivity in shock group was significantly lower than that of sham group, MLDL or PSML drainage enhanced the calcium sensitivity. After incubating with PKG regulators in shock+ligation and shock+drainage groups, the PKG agonist 8Br-cGMP reduced the contractility of vascular rings to gradient calcium ions and Emax and the PKG inhibitor agonist KT5823 elevated the calcium sensitivity significantly. CONCLUSION:
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Authors | Zi-Gang Zhao, Yan-Ling Wei, Chun-Yu Niu, Yu-Ping Zhang, Li-Min Zhang, Li-Na Jiang |
Journal | Acta cirurgica brasileira
(Acta Cir Bras)
Vol. 28
Issue 7
Pg. 537-42
(Jul 2013)
ISSN: 1678-2674 [Electronic] Brazil |
PMID | 23842936
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cyclic GMP-Dependent Protein Kinases
- Calcium
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Topics |
- Animals
- Blotting, Western
- Calcium
(analysis, metabolism)
- Cyclic GMP-Dependent Protein Kinases
(analysis, physiology)
- Enzyme-Linked Immunosorbent Assay
- Male
- Mesenteric Artery, Superior
(metabolism, physiopathology)
- Muscle Contraction
- Random Allocation
- Rats
- Rats, Wistar
- Shock, Hemorrhagic
(metabolism, physiopathology)
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