Study of the biological functions of mucin type core 3 O-glycans.

Abstract

Core 3 O-glycan is very short glycan structure which is composed of one N-acetylglucosamine and one N-acetylgalactosamine. The core 3 O-glycan structure is synthesized by core3 synthase (beta 1, 3-N-acetylglucosaminyltransferase 6) using UDP-N-acetylglucosamine as substrate. We revealed that the core 3 O-glycan structure modulates prostate cancer formation and gastrointestinal cell differentiation through regulating the heterodimerization of α2β1 integrin and cell surface expression of differentiation marker proteins respectively. This chapter describes the way to determine the functions of core 3 O-glycan in tumor formation and gastrointestinal cell differentiation.

• Authors: Seung Ho Lee, Minoru Fukuda
• Journal: Methods in molecular biology (Clifton, N.J.) (Methods Mol Biol) Vol. 1022 Pg. 41-50 ( 2013) ISSN: 1940-6029 [Electronic] United States
• PMID: 23765652 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Integrin alpha2beta1
• Polysaccharides
• N-Acetylglucosaminyltransferases
• UDP-GlcNAc GalNAc-peptide beta1,3-N-acetylglucosaminyltransferase

Topics

• Animals
• Cell Differentiation
• Cell Line, Tumor
• Gastrointestinal Tract (cytology)
• Humans
• Immunohistochemistry (methods)
• Integrin alpha2beta1 (metabolism)
• Male
• Mice
• Mice, Inbred BALB C
• N-Acetylglucosaminyltransferases (metabolism)
• Polysaccharides (chemistry, metabolism)
• Prostate (metabolism)
• Prostatic Neoplasms (metabolism)
• Protein Multimerization