Protective effect of esculentoside A on lipopolysaccharide-induced acute lung injury in mice.

Abstract

BACKGROUND:

Esculentoside A (EsA) is a saponin isolated from the Chinese herb Phytolacca esculenta. In our study, we sought to investigate the protective effects of EsA on lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice.

MATERIALS AND METHODS:

To determine the effects of EsA on the reduction of histopathologic changes in mice with ALI, inflammatory cell count in bronchoalveolar lavage fluid (BALF) and lung wet-to-dry weight ratio were measured in LPS-challenged mice, and lung histopathologic changes observed via paraffin section were assessed. Next, cytokine production induced by LPS in BALF was measured by enzyme-linked immunosorbent assay. To further study the mechanism of EsA protective effects on ALI, IκBa, p38, and extracellular signal receptor-activated kinase pathways were investigated in lung tissue of mice with ALI.

RESULTS:

In the present investigation, EsA showed marked effects by reducing inflammatory infiltration, thickening of the alveolar wall, and pulmonary congestion. Levels of tumor necrosis factor α and interleukin 6 elevated by LPS were significantly decreased in BALF in EsA-pretreated ALI model. Furthermore, EsA significantly suppressed phosphorylation of IκBa, p38, and extracellular signal receptor-activated kinase.

CONCLUSIONS:

Taken together, our results suggest that EsA suppressed inflammatory responses in LPS-induced ALI through inhibition of the nuclear factor kappa B and mitogen activated protein kinase signaling pathways. EsA may be a promising potential preventive agent for ALI treatment.

• Authors: Wei-ting Zhong, Lan-xiang Jiang, Jing-yuan Wei, An-na Qiao, Miao-miao Wei, Lanan-Wassy Soromou, Xian-xing Xie, Xuan Zhou, Xin-xin Ci, Da-cheng Wang
• Journal: The Journal of surgical research (J Surg Res) Vol. 185 Issue 1 Pg. 364-72 (Nov 2013) ISSN: 1095-8673 [Electronic] United States
• PMID: 23764313 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Elsevier Inc. All rights reserved.

Chemical References

• Drugs, Chinese Herbal
• I-kappa B Proteins
• Lipopolysaccharides
• Nfkbia protein, mouse
• Saponins
• NF-KappaB Inhibitor alpha
• esculentoside A
• Oleanolic Acid

Topics

• Acute Lung Injury (chemically induced, immunology, prevention & control)
• Animals
• Bronchoalveolar Lavage Fluid (cytology, immunology)
• Disease Models, Animal
• Drugs, Chinese Herbal (chemistry, pharmacology)
• I-kappa B Proteins (immunology)
• Lipopolysaccharides (pharmacology)
• Lung (drug effects, immunology, metabolism)
• MAP Kinase Signaling System (drug effects, immunology)
• Male
• Mice
• Mice, Inbred BALB C
• NF-KappaB Inhibitor alpha
• Oleanolic Acid (analogs & derivatives, chemistry, pharmacology)
• Pulmonary Edema (chemically induced, immunology, prevention & control)
• Saponins (chemistry, pharmacology)