Abstract | INTRODUCTION: MATERIALS AND METHODS: Platelets and platelet-free plasma were obtained from healthy, adult subjects. The dose-dependent effects of histone H4 on platelet aggregation were studied by optical aggregometry. The effects of native and albumin-depleted plasma (prepared by affinity chromatography) on histone-induced platelet aggregation were also assessed. The effects of normal and surface-neutralized albumin (through modification of carboxyl groups) on histone-induced platelet activation and aggregation were evaluated using flow cytometry and aggregometry. RESULTS:
Histone H4 induced platelet aggregation in a dose-dependent manner. This histone-induced platelet aggregation was inhibited by both plasma and human serum albumin in a dose-dependent fashion. Furthermore, depletion of albumin from plasma reduced its ability to inhibit aggregation. Finally, surface neutralization of albumin decreased its ability to inhibit histone-induced activation and aggregation. DISCUSSION: These data suggest that normal albumin serves a role in preventing histone-induced platelet aggregation in a charge-dependent manner.
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Authors | Fong W Lam, Miguel A Cruz, Hon-Chiu E Leung, Kathan S Parikh, C Wayne Smith, Rolando E Rumbaut |
Journal | Thrombosis research
(Thromb Res)
Vol. 132
Issue 1
Pg. 69-76
(Jul 2013)
ISSN: 1879-2472 [Electronic] United States |
PMID | 23673386
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
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Copyright | Copyright © 2013 Elsevier Ltd. All rights reserved. |
Chemical References |
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Topics |
- Adult
- Blood Platelets
(cytology, metabolism)
- Histones
(metabolism)
- Humans
- Platelet Aggregation
- Platelet Function Tests
- Serum Albumin
(metabolism)
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