Abstract | ETHNOPHARMACOLOGICAL RELEVANCE: Centipeda minima (L.) is traditionally used in Chinese folk medicine for the treatments of rhinitis, sinusitis, relieving pain, reducing swelling, and treating cancer for a long history in Taiwan. However, there is no scientific evidence which supports the use in the literature. AIM OF THE STUDY: The aim of this study was to evaluate the antioxidant and anti-inflammatory activities of the aqueous extract of Centipeda minima (ACM). MATERIALS AND METHODS: RESULTS: ACM showed the highest TEAC and DPPH radical scavenging activities, respectively. ACM also had highest contents of polyphenol and flavonoid contents. We evaluated that ACM and the reference compound of protocatechualdehyde and caffeic acid decreased the LPS-induced NO production in RAW264.7 cells. Administration of ACM showed a concentration dependent inhibition on paw edema development after Carr treatment in mice. The anti-inflammatory effects of ACM could be via NO, TNF-α, and IL-1β suppressions and associated with the increase in the activities of antioxidant enzymes. Western blotting revealed that ACM decreased Carr-induced inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expressions. CONCLUSIONS: Anti-inflammatory mechanisms of ACM might be correlated to the decrease in the level of Malondialdehyde (MDA), iNOS, and COX-2 via increasing the activities of CAT, SOD, and GPx in the edema paw. Overall, the results showed that ACM demonstrated antioxidant and anti-inflammatory activity, which supports previous claims of the traditional use for inflammation and pain.
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Authors | Shyh-Shyun Huang, Chuan-Sung Chiu, Tsung-Hui Lin, Min-Min Lee, Chao-Ying Lee, Shu-Jen Chang, Wen-Chi Hou, Guan-Jhong Huang, Jeng-Shyan Deng |
Journal | Journal of ethnopharmacology
(J Ethnopharmacol)
Vol. 147
Issue 2
Pg. 395-405
(May 20 2013)
ISSN: 1872-7573 [Electronic] Ireland |
PMID | 23506988
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2013 Elsevier Ireland Ltd. All rights reserved. |
Chemical References |
- Anti-Inflammatory Agents
- Antioxidants
- Interleukin-1beta
- Plant Extracts
- Tumor Necrosis Factor-alpha
- Nitric Oxide
- Carrageenan
- Catalase
- Glutathione Peroxidase
- Nitric Oxide Synthase Type II
- Nos2 protein, mouse
- Ptgs2 protein, mouse
- Cyclooxygenase 2
- Superoxide Dismutase
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Topics |
- Animals
- Anti-Inflammatory Agents
(pharmacology, therapeutic use)
- Antioxidants
(pharmacology, therapeutic use)
- Asteraceae
- Carrageenan
- Catalase
(metabolism)
- Cell Line
- Cell Survival
(drug effects)
- Cyclooxygenase 2
(metabolism)
- Edema
(chemically induced, drug therapy, metabolism, pathology)
- Glutathione Peroxidase
(metabolism)
- Interleukin-1beta
(blood)
- Male
- Mice
- Mice, Inbred ICR
- Nitric Oxide
(metabolism)
- Nitric Oxide Synthase Type II
(metabolism)
- Phytotherapy
- Plant Extracts
(pharmacology, therapeutic use)
- Superoxide Dismutase
(metabolism)
- Tumor Necrosis Factor-alpha
(blood)
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