Abstract |
To investigate influence of general anesthesia on immunological anti- tumor activity, the natural killer (NK) cytotoxic activity of peripheral lymphocytes (PBLs) was measured in 7 dogs anesthetized for 3 hr with isoflurane following propofol-induction ( anesthesia group) and 6 dogs without anesthesia (control group). Blood samples were collected before (baseline) and 24, 120 and 192 hr after the anesthesia. The PBLs were isolated via centrifugation with Ficoll- Hypaque solution (density, 1.073), and adherent cells were removed. The NK cytotoxic activity of the isolated PBLs against canine thyroid cancer cells was detected by the colorimetric rose Bengal assay. Significant decrease in the NK cytotoxic activity was observed at 24 hr after the anesthesia, compared with the baseline values and the control group. The NK cytotoxic activities were recovered to the baseline values until 120 hr after the anesthesia. The general anesthesia with isoflurane following propofol-induction decreased the NK cytotoxic activities of PBLs in dogs. This finding has a clinical relevance to the risk of tumor recurrence or metastasis induced by the suppression of immunological anti- tumor activity after general anesthesia in dogs. The results further emphasized the importance of the need to evaluate immune suppression following general anesthesia in animals.
|
Authors | Takuma Miyata, Takanori Kodama, Rie Honma, Yoshinori Nezu, Yasuji Harada, Takuya Yogo, Yasushi Hara, Masahiro Tagawa |
Journal | The Journal of veterinary medical science
(J Vet Med Sci)
Vol. 75
Issue 7
Pg. 917-21
(Jul 31 2013)
ISSN: 1347-7439 [Electronic] Japan |
PMID | 23446140
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Rose Bengal
- Isoflurane
- Propofol
|
Topics |
- Anesthesia
(methods, veterinary)
- Animals
- Cell Line, Tumor
- Centrifugation
(veterinary)
- Dogs
- Female
- Flow Cytometry
(veterinary)
- Isoflurane
(pharmacology)
- Killer Cells, Natural
(drug effects, immunology)
- Lymphocyte Subsets
(immunology)
- Male
- Propofol
(pharmacology)
- Rose Bengal
- Time Factors
|