CUL3 and NRF2 mutations confer an NRF2 activation phenotype in a sporadic form of papillary renal cell carcinoma.

Abstract

Sustained activation of the stress-regulated transcription factor NRF2 (NFE2L2) is a prominent feature of many types of cancer, implying that mutations driving NRF2 may be important to tumor progression. In hereditary type 2 papillary renal cell carcinoma (PRCC2, also known as hereditary leiomyomatosis and renal cell cancer), NRF2 activation is a direct consequence of the accumulation of intracellular fumarate, a result of fumarate hydratase (FH) inactivation, but it is not clear how NRF2 may be activated in sporadic forms of PRCC2. Here we show that somatic mutations in NRF2, CUL3, and SIRT1 are responsible for driving the NRF2 activation phenotype in sporadic PRCC2. Transcriptome sequencing revealed the expression pattern of mutant alleles of NRF2, CUL3, and SIRT1 and also confirmed NRF2 activation in clinical specimens. Our results show a convergence in somatic mutations in sporadic PRCC2 with FH mutation in hereditary PRCC2.

• Authors: Aikseng Ooi, Karl Dykema, Asif Ansari, David Petillo, John Snider, Richard Kahnoski, John Anema, David Craig, John Carpten, Bin-Tean Teh, Kyle A Furge
• Journal: Cancer research (Cancer Res) Vol. 73 Issue 7 Pg. 2044-51 (Apr 01 2013) ISSN: 1538-7445 [Electronic] United States
• PMID: 23365135 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: ©2013 AACR.

Chemical References

• Biomarkers, Tumor
• CUL3 protein, human
• Cullin Proteins
• Intracellular Signaling Peptides and Proteins
• KEAP1 protein, human
• Kelch-Like ECH-Associated Protein 1
• NF-E2-Related Factor 2
• NFE2L2 protein, human
• SIRT1 protein, human
• Sirtuin 1

Topics

• Amino Acid Sequence
• Biomarkers, Tumor (genetics, metabolism)
• Blotting, Western
• Carcinoma, Renal Cell (genetics, metabolism, pathology)
• Cells, Cultured
• Cullin Proteins (genetics, metabolism)
• Gene Expression Profiling
• High-Throughput Nucleotide Sequencing
• Humans
• Immunoenzyme Techniques
• Intracellular Signaling Peptides and Proteins (metabolism)
• Kelch-Like ECH-Associated Protein 1
• Kidney Neoplasms (genetics, metabolism, pathology)
• Kidney Tubules, Proximal (cytology, metabolism)
• Molecular Sequence Data
• Mutation (genetics)
• NF-E2-Related Factor 2 (genetics, metabolism)
• Oligonucleotide Array Sequence Analysis
• Phenotype
• Sequence Homology, Amino Acid
• Sirtuin 1 (metabolism)