Abstract | BACKGROUND AND PURPOSE: Severe intraventricular hemorrhage (IVH) in premature infants and the ensuing posthemorrhagic hydrocephalus cause significant mortality and neurological disabilities, and there are currently no effective therapies. This study determined whether intraventricular transplantation of human umbilical cord blood-derived mesenchymal stem cells prevents posthemorrhagic hydrocephalus development and attenuates brain damage after severe IVH in newborn rats. METHODS: To induce severe IVH, 100 μL of blood was injected into each lateral ventricle of postnatal day 4 (P4) Sprague-Dawley rats. Human umbilical cord blood-derived mesenchymal stem cells or fibroblasts (1 × 10(5)) were transplanted intraventricularly under stereotaxic guidance at P6. Serial brain MRI and behavioral function tests, such as the negative geotaxis test and rotarod test, were performed. At P32, brain tissue and cerebrospinal fluid were obtained for histological and biochemical analyses. RESULTS: CONCLUSIONS: Intraventricular transplantation of umbilical cord blood-derived mesenchymal stem cells significantly attenuated the posthemorrhagic hydrocephalus and brain injury after IVH. This neuroprotective mechanism appears to be mediated by the anti-inflammatory effects of these cells.
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Authors | So Yoon Ahn, Yun Sil Chang, Dong Kyung Sung, Se In Sung, Hye Soo Yoo, Jung Hee Lee, Won Il Oh, Won Soon Park |
Journal | Stroke
(Stroke)
Vol. 44
Issue 2
Pg. 497-504
(Feb 2013)
ISSN: 1524-4628 [Electronic] United States |
PMID | 23287782
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Topics |
- Animals
- Animals, Newborn
- Cells, Cultured
- Cerebral Hemorrhage
(pathology, surgery)
- Female
- Humans
- Hydrocephalus
(pathology, prevention & control)
- Infant, Newborn
- Lateral Ventricles
(pathology, surgery)
- Male
- Mesenchymal Stem Cell Transplantation
(methods)
- Pregnancy
- Rats
- Rats, Sprague-Dawley
- Severity of Illness Index
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