Abstract | OBJECTIVE: METHODS: In AQP4-knockout (KO) and wild-type mice, focal cerebral ischemia was induced by 30 min of middle cerebral arterial occlusion (MCAO). Ischemic neuronal injury and cellular inflammatory responses, as well as the expression and localization of cysteinyl leukotriene CysLT(2) and CysLT(1) receptors, were determined at 24 and 72 h after MCAO. RESULTS: AQP4-KO mice showed more neuronal loss, more severe microglial activation and neutrophil infiltration, but less astrocyte proliferation in the brain after MCAO than wild-type mice. In addition, the protein levels of both CysLT(1) and CysLT(2) receptors were up-regulated in the ischemic brain, and the up-regulation was more pronounced in AQP4-KO mice. The CysLT(1) and CysLT(2) receptors were primarily localized in neurons, microglia and neutrophils; those localized in microglia and neutrophils were enhanced in AQP4-KO mice. CONCLUSION: AQP4 may play an inhibitory role in postischemic inflammation.
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Authors | Wen-Zhen Shi, Chun-Zhen Zhao, Bing Zhao, Qiao-Juan Shi, Li-Hui Zhang, Yan-Fang Wang, San-Hua Fang, Yun-Bi Lu, Wei-Ping Zhang, Er-Qing Wei |
Journal | Neuroscience bulletin
(Neurosci Bull)
Vol. 28
Issue 6
Pg. 680-92
(Dec 2012)
ISSN: 1995-8218 [Electronic] Singapore |
PMID | 23132680
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Aquaporin 4
- Receptors, Leukotriene
- leukotriene D4 receptor
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Topics |
- Animals
- Aquaporin 4
(deficiency, genetics)
- Astrocytes
(metabolism)
- Blotting, Western
- Brain Ischemia
(metabolism, pathology)
- Cell Count
- Immunohistochemistry
- Infarction, Middle Cerebral Artery
(metabolism, pathology)
- Inflammation
(metabolism, pathology)
- Leukocytes
(metabolism)
- Mice
- Mice, Knockout
- Microglia
(physiology)
- Neutrophils
(metabolism)
- Real-Time Polymerase Chain Reaction
- Receptors, Leukotriene
(biosynthesis)
- Up-Regulation
- Water Intoxication
(metabolism)
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