Immunotherapy against HPV16/18 generates potent TH1 and cytotoxic cellular immune responses.

Abstract

Despite the development of highly effective prophylactic vaccines against human papillomavirus (HPV) serotypes 16 and 18, prevention of cervical dysplasia and cancer in women infected with high-risk HPV serotypes remains an unmet medical need. We report encouraging phase 1 safety, tolerability, and immunogenicity results for a therapeutic HPV16/18 candidate vaccine, VGX-3100, delivered by in vivo electroporation (EP). Eighteen women previously treated for cervical intraepithelial neoplasia grade 2 or 3 (CIN2/3) received a three-dose (intramuscular) regimen of highly engineered plasmid DNA encoding HPV16 and HPV18 E6/E7 antigens followed by EP in a dose escalation study (0.3, 1, and 3 mg per plasmid). Immunization was well tolerated with reports of mild injection site reactions and no study-related serious or grade 3 and 4 adverse events. No dose-limiting toxicity was noted, and pain was assessed by visual analog scale, with average scores decreasing from 6.2/10 to 1.4 within 10 min. Average peak interferon-γ enzyme-linked immunospot magnitudes were highest in the 3 mg cohort in comparison to the 0.3 and 1 mg cohorts, suggesting a trend toward a dose effect. Flow cytometric analysis revealed the induction of HPV-specific CD8(+) T cells that efficiently loaded granzyme B and perforin and exhibited full cytolytic functionality in all cohorts. These data indicate that VGX-3100 is capable of driving robust immune responses to antigens from high-risk HPV serotypes and could contribute to elimination of HPV-infected cells and subsequent regression of the dysplastic process.

• Authors: Mark L Bagarazzi, Jian Yan, Matthew P Morrow, Xuefei Shen, R Lamar Parker, Jessica C Lee, Mary Giffear, Panyupa Pankhong, Amir S Khan, Kate E Broderick, Christine Knott, Feng Lin, Jean D Boyer, Ruxandra Draghia-Akli, C Jo White, J Joseph Kim, David B Weiner, Niranjan Y Sardesai
• Journal: Science translational medicine (Sci Transl Med) Vol. 4 Issue 155 Pg. 155ra138 (Oct 10 2012) ISSN: 1946-6242 [Electronic] United States
• PMID: 23052295 (Publication Type: Clinical Trial, Phase I, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Viral
• Papillomavirus Vaccines
• VGX-3100
• Vaccines, DNA

Topics

• Adult
• Antigens, Viral (genetics, immunology, metabolism)
• Electroporation
• Female
• Human papillomavirus 16 (immunology)
• Human papillomavirus 18 (immunology)
• Humans
• Immunotherapy (adverse effects, methods)
• Papillomavirus Vaccines (immunology, therapeutic use)
• Vaccines, DNA (immunology, therapeutic use)
• Uterine Cervical Dysplasia (immunology, therapy)