Adenosine receptor stimulation by polynucleotides (PDRN) reduces inflammation in experimental periodontitis.

Abstract

AIM:

Adenosine receptors modulate inflammation in periodontal tissues. No data are available regarding the effects of adenosine A(2A) receptor stimulation in experimental periodontitis (EPD). The aim of this study was to investigate the effects of polynucleotides (also known as polydeoxyribonucleotide, PDRN), a ligand of A(2A) receptor, in EPD in rats.

MATERIALS AND METHODS:

EPD was induced ligating the cervix of the lower left first molar. Sham-EPD had no ligature. After 7 days, EPD animals were randomized to a daily treatment with vehicle gel or 0.75% PDRN gel or PDRN gel with a specific A(2A) antagonist (DMPX). Treatments lasted 7 days. Animals were then euthanized and the periodontium and surrounding gingival tissue were excised for histological evaluation and bio-molecular analysis of inflammatory (p-JNK, p-ERK, TNF-α, IL-6, HMGB-1) and apoptotic proteins (BAX and Bcl-2).

RESULTS:

Vehicle-treated EPD rats showed severe inflammatory infiltrate in both gingival and periodontal ligament, as well as an enhanced expression of p-JNK, p-ERK, TNF-α, IL-6, HMGB-1 and BAX and a reduction in Bcl-2. PDRN gel restored the histological features, blunted inflammatory and apoptotic proteins expression and preserved Bcl-2 expression. DMPX abrogated PDRN positive effects.

CONCLUSION:

Our data suggest that adenosine receptor stimulation by PDRN might represent a new therapeutic strategy for periodontitis.

• Authors: Alessandra Bitto, Giacomo Oteri, Michele Pisano, Francesca Polito, Natasha Irrera, Letteria Minutoli, Francesco Squadrito, Domenica Altavilla
• Journal: Journal of clinical periodontology (J Clin Periodontol) Vol. 40 Issue 1 Pg. 26-32 (Jan 2013) ISSN: 1600-051X [Electronic] United States
• PMID: 23033941 (Publication Type: Journal Article)
• Copyright: © 2012 John Wiley & Sons A/S.

Chemical References

• Adenosine A2 Receptor Antagonists
• HMGB1 Protein
• Inflammation Mediators
• Interleukin-6
• Polydeoxyribonucleotides
• Proto-Oncogene Proteins c-bcl-2
• Receptors, Purinergic P1
• Tumor Necrosis Factor-alpha
• bcl-2-Associated X Protein
• 3,7-dimethyl-1-propargylxanthine
• Extracellular Signal-Regulated MAP Kinases
• JNK Mitogen-Activated Protein Kinases
• Theobromine

Topics

• Adenosine A2 Receptor Antagonists (pharmacology)
• Alveolar Bone Loss (metabolism)
• Animals
• Extracellular Signal-Regulated MAP Kinases (metabolism)
• HMGB1 Protein (metabolism)
• Inflammation Mediators (pharmacology)
• Interleukin-6 (metabolism)
• JNK Mitogen-Activated Protein Kinases (metabolism)
• Male
• Periodontitis (metabolism)
• Polydeoxyribonucleotides (pharmacology)
• Proto-Oncogene Proteins c-bcl-2 (metabolism)
• Random Allocation
• Rats
• Rats, Sprague-Dawley
• Receptors, Purinergic P1 (drug effects)
• Theobromine (analogs & derivatives, pharmacology)
• Tumor Necrosis Factor-alpha (metabolism)
• bcl-2-Associated X Protein (metabolism)