Abstract | OBJECTIVE: The present study investigated the effects of ovarian hormone depletion and estrogen administration on ischemia/reperfusion (I/R)-induced bladder damage in female rabbits. METHODS: RESULTS: I/R surgical procedure decreased bladder contractile responses by 30% to 50%. Ovarian hormone depletion further reduced bladder contractile function by 45% to 55% compared with the I/R group members that retained their ovaries. Moreover, I/R surgical procedure significantly decreased intramural neurofilament staining by two thirds compared with the control group. Estrogen replacement after ovariectomy significantly increased the density of nerve terminals. In addition, the expression of transforming growth factor-β and fibronectin increased twofold and fivefold after I/R, respectively. Ovarian hormone depletion further increased the expression of these inflammatory and fibrosis markers. Ovariectomy significantly exacerbated oxidative damage, whereas estrogen replacement diminished oxidative stress to a level approaching that of the control group. CONCLUSIONS:
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Authors | Shu-Mien Chuang, Cheng-Yu Long, Rong-Jyh Lin, Keh-Min Liu, Robert M Levin, Chao-Yuan Chang, Ya-Wen Ho, Wen-Jeng Wu, Wei-Chiao Chang, Yung-Shun Juan |
Journal | Menopause (New York, N.Y.)
(Menopause)
Vol. 20
Issue 2
Pg. 209-17
(Feb 2013)
ISSN: 1530-0374 [Electronic] United States |
PMID | 23010881
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Fibronectins
- Transforming Growth Factor beta
- Estradiol
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Topics |
- Animals
- Estradiol
(administration & dosage)
- Estrogen Replacement Therapy
- Female
- Fibronectins
(genetics)
- Gene Expression
(drug effects)
- Injections, Intramuscular
- Muscle Contraction
(drug effects)
- Ovariectomy
- Rabbits
- Reperfusion Injury
(complications)
- Transforming Growth Factor beta
(genetics)
- Urinary Bladder
(blood supply)
- Urinary Bladder Diseases
(etiology, pathology, prevention & control)
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