Many scientific studies have shown that
laminarin has anti-
tumor effects, but the anti-
tumor mechanism was unclear. The purpose of this study was to investigate the effect of
laminarin on the induction of apoptosis in human
colon cancer LOVO cells and the molecular mechanism involved. LOVO cells were treated with different concentrations of
laminarin at different times. Morphology observations were performed to determine the effects of
laminarin on apoptosis of LOVO cells. Flow cytometry (FCM) was used to detect the level of intracellular
reactive oxygen species (ROS) and pH.
Laser scanning confocal microscope (LSCM) was used to analyze intracellular
calcium ion concentration, mitochondrion permeability transition pore (
MPTP) and mitochondrial membrane potential (
MMP). Western blotd were performed to analyze the expressions of Cyt-C,
Caspase-9 and -3. The results showed the apoptosis morphology, which showed cell protuberance, concentrated cytoplasm and apoptotic bodies, was obvious after 72 h treatment.
Laminarin treatment for 24 h increased the intracellular level of ROS and Ca²⁺; decreased pH value; activated intracellular
MPTP and decreased
MMP in dose-dependent manners. It also induced the release of Cyt-C and the activation of
Caspase-9 and -3. In conclusion,
laminarin induces LOVO cell apoptosis through a mitochondrial pathway, suggesting that it could be a potent agent for
cancer prevention and treatment.