Increased levels of acute-phase inflammatory proteins in plasma of patients with sporadic CJD.

Abstract

OBJECTIVE:

Screening plasma samples from patients with sporadic Creutzfeldt-Jakob disease (CJD) to discover diagnostic biomarkers.

METHODS:

Plasma samples were collected from 17 patients with sporadic CJD, 17 patients with Alzheimer disease (AD), and 20 healthy subjects. A 2-phase screening was carried out using quantitative protein mass spectrometry. The putative sporadic CJD biomarkers were then validated independently by immunoturbidimetry.

RESULTS:

Mass spectrometry uncovered 7 candidate sporadic CJD protein biomarkers, all belonging to the acute-phase response. Highly significant increases of these markers in patients with sporadic CJD, compared with healthy subjects and patients with AD, was confirmed by immunoturbidimetry.

CONCLUSIONS:

The increase in plasma levels of a related set of acute-phase reactants in patients with sporadic CJD is a novel finding that suggests new pathogenetic hypotheses. The possible value of this set of proteins as biomarkers in the diagnosis of sporadic CJD or for blood/tissue donor screening remains to be further explored and validated in larger studies.

• Authors: Federica Fratini, Serena Principe, Maria Puopolo, Anna Ladogana, Anna Poleggi, Paola Piscopo, Giuseppe Bruno, Silvano Castrechini, Roberto Pascone, Annamaria Confaloni, Luisa Minghetti, Franco Cardone, Maurizio Pocchiari, Marco Crescenzi
• Journal: Neurology (Neurology) Vol. 79 Issue 10 Pg. 1012-8 (Sep 04 2012) ISSN: 1526-632X [Electronic] United States
• PMID: 22855863 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Acute-Phase Proteins
• Biomarkers

Topics

• Acute-Phase Proteins (metabolism)
• Aged
• Aged, 80 and over
• Alzheimer Disease (blood)
• Biomarkers (blood)
• Creutzfeldt-Jakob Syndrome (blood, diagnosis)
• Female
• Humans
• Male
• Middle Aged