Black tea polyphenols reverse epithelial-to-mesenchymal transition and suppress cancer invasion and proteases in human oral cancer cells.

Abstract

Epithelial-to-mesenchymal transition (EMT) in cancer cells is considered to be a prerequisite for acquiring invasive/migratory phenotype and subsequent metastasis. This study provides molecular evidence associated with the antimetastatic effect of black tea polyphenol extracts (BTE), which contain polyphenols including gallic acid, gallocatechin, catechin, epigallocatechin-3-gallate, epicatechin-3-gallate, and theaflavin 3,3'-digallate, in an an oral squamous cell culture system by showing a nearly complete inhibition on the invasion (p < 0.001) of SCC-4 cells via reduced activities of MMP-2 (p < 0.001) and u-PA (p < 0.001). Immunoblot was performed to find that BTE could induce up-regulation of epithelial markers such as E-cadherin and inhibit mesenchymal markers such as snail-1 and vimentin. BTE inhibited p-FAK and p-paxillin, indicating the anti-EMT effect of BTE in oral squamous cell carcinoma. BTE was evidenced by its inhibition of the tumor growth of SCC-4 cells via cancer cell xenografted nude mice mode. These results suggested that BTE could reduce invasion by reversing EMT in human oral cancer cells.

• Authors: Yu-Chao Chang, Pei-Ni Chen, Shu-Chen Chu, Chin-Yin Lin, Wu-Hsien Kuo, Yih-Shou Hsieh
• Journal: Journal of agricultural and food chemistry (J Agric Food Chem) Vol. 60 Issue 34 Pg. 8395-403 (Aug 29 2012) ISSN: 1520-5118 [Electronic] United States
• PMID: 22827697 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cadherins
• Polyphenols
• Protease Inhibitors
• Vimentin
• Urokinase-Type Plasminogen Activator
• Matrix Metalloproteinase 2

Topics

• Animals
• Cadherins (metabolism)
• Camellia sinensis (chemistry)
• Cell Movement (drug effects)
• Epithelial-Mesenchymal Transition (drug effects, physiology)
• Humans
• Matrix Metalloproteinase 2 (metabolism)
• Mice
• Mice, Nude
• Mouth Neoplasms (drug therapy, metabolism, pathology)
• Neoplasms, Squamous Cell (drug therapy, metabolism, pathology)
• Polyphenols (pharmacology)
• Promoter Regions, Genetic (drug effects)
• Protease Inhibitors (pharmacology)
• Tumor Cells, Cultured
• Urokinase-Type Plasminogen Activator (genetics, metabolism)
• Vimentin (antagonists & inhibitors, metabolism)
• Xenograft Model Antitumor Assays