Glutamine modulates sepsis-induced changes to intestinal intraepithelial γδT lymphocyte expression in mice.

Abstract

This study investigated the effect of glutamine (GLN) on intestinal intraepithelial lymphocyte (IEL) γδT-cell cytokines and immune regulatory factor gene expressions in a mouse model of polymicrobial sepsis. Mice were randomly assigned to a normal group, a sepsis with saline (SS) group, or a sepsis with GLN (SG) group. All mice were fed a chow diet. Sepsis was induced by cecal ligation and puncture (CLP). The SS group was injected with saline, and the SG group was given 0.75 g GLN/kg body weight once via a tail vein 1 h after CLP. Septic mice were killed 12 h after CLP, and IEL γδT cells of the animals were isolated for further analysis. Results showed that compared with normal mice, sepsis resulted in lower IEL γδT-cell percentage and higher messenger RNA expressions of interferon γ, tumor necrosis factor α, interleukin 4 (IL-4), IL-13, IL-17, retinoid acid receptor-related orphan receptor γt, and complement 5a receptor by IEL γδT cells. These immunomodulatory mediator genes exhibited decreases, whereas IL-7 receptor expression increased in IEL γδT cells in septic mice with GLN administration. Annexin V/7-amino-actinomycin D stain revealed significantly lower rates of apoptosis, and IEL γδT-cell percentage was higher in the SG group. The histological findings also showed that damage to intestinal epithelial cells was less severe in the SG group. These results indicated that a single dose of GLN administered as treatment after the initiation of sepsis prevented apoptosis of IEL γδT cells and downregulated γδT cell-expressed inflammatory mediators that may consequently ameliorate the severity of sepsis-induced intestinal epithelial injury.

• Authors: Wan-Yun Lee, Ya-Mei Hu, Tsui-Ling Ko, Sung-Ling Yeh, Chiu-Li Yeh
• Journal: Shock (Augusta, Ga.) (Shock) Vol. 38 Issue 3 Pg. 288-93 (Aug 2012) ISSN: 1540-0514 [Electronic] United States
• PMID: 22777117 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cytokines
• RNA, Messenger
• Transcription Factors
• Glutamine

Topics

• Animals
• Apoptosis (drug effects)
• Cecal Diseases (immunology, pathology)
• Cytokines (metabolism)
• Down-Regulation
• Gene Expression
• Glutamine (pharmacology)
• Intestinal Mucosa (pathology)
• Male
• Mice
• Mice, Inbred C57BL
• RNA, Messenger (metabolism)
• Random Allocation
• Sepsis (immunology, pathology)
• T-Lymphocytes (drug effects, immunology)
• Transcription Factors (metabolism)