Abstract |
Eleven derivatives from Antrodia camphorata were isolated in order to evaluate their selective cytotoxicity toward 14 types of human cancer cell and two non-transformed cell types. Among these triterpenoids, methyl antcinate A (MAA) exhibited the most potent spectrum of anticancer effects in KB cells, four different oral cancer cell lines (TSCCa, GNM, OC-2, and OEC-M1), Panc-1, BT474, PC-3, OVCAR-3, HeLa, and U2OS cells with high selectivity indices (CC(50)/IC(50)). The expression of B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X protein (Bax), and poly(ADP-ribose) polymerase (PARP) of PC-3 cells tested by western blotting suggested that MAA exerts cell death through the caspase-dependent cascade and the Bax-mediated mitochondrial apoptotic pathway, not only on liver and oral cancer cells but on other types as well, including prostate cancer, in a dose-dependent manner. In addition to MAA, methyl antcinate B, dehydroeburicoic acid, and 15α-acetyl-dehydrosulfurenic acid also exhibited significant selective cytotoxic effects to respective cancer cells. Modifications of these triterpenoids may lead to the development of more potent anticancer drugs.
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Authors | Yi-Pang Lee, Wan-Chi Tsai, Chih-Jan Ko, Yerra Koteswara Rao, Chiung-Ru Yang, Dar-Ren Chen, Ming-Hui Yang, Chi-Chiang Yang, Yew-Min Tzeng |
Journal | Anticancer research
(Anticancer Res)
Vol. 32
Issue 7
Pg. 2727-34
(Jul 2012)
ISSN: 1791-7530 [Electronic] Greece |
PMID | 22753732
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents, Phytogenic
- Plant Extracts
- Triterpenes
- Caspase 3
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Topics |
- Antineoplastic Agents, Phytogenic
(chemistry, isolation & purification, pharmacology)
- Antrodia
(chemistry)
- Apoptosis
(drug effects)
- Caspase 3
(metabolism)
- Cell Line, Tumor
- Humans
- KB Cells
- Mouth Neoplasms
(drug therapy)
- Plant Extracts
(isolation & purification, pharmacology)
- Triterpenes
(isolation & purification, pharmacology)
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