Abstract | CONTEXT: OBJECTIVE: To assess the 3' UTR polyA tract of EGFR in both endometrial and colorectal carcinomas and the mutational status of EGFR downstream pathways. DESIGN: Ninety-eight colorectal carcinomas and 47 endometrial carcinomas were included. EGFR 3' UTR polyA status was detected by capillary electrophoresis and Sanger sequencing. EGFR gene expression, EGFR copy numbers, and KRAS and BRAF mutation status were analyzed accordingly. RESULTS: The 3' UTR polyA tract was deleted in 18 of 23 (78%) MSI-H versus 0 of 24 microsatellite-stable endometrial carcinomas (P < .001). Similar observations were seen in colorectal carcinomas, in which 29 of 36 (81%) MSI-H, 1 of 62 (1.6%) microsatellite instability-low, and none of the microsatellite-stable tumors harbored the deletion (P < .001). A moderate increase in EGFR mRNA level was observed in endometrial carcinomas with 3' UTR polyA deletions versus those with wild-type polyA tract. Amplification of the EGFR gene was not observed. Deletions in polyA tract do not seem to affect the frequency of KRAS and BRAF mutations. CONCLUSIONS: Deletions of EGFR 3' UTR polyA are frequent in endometrial and colorectal carcinomas, are confined almost exclusively to MSI-H tumors, and do not affect KRAS and BRAF mutations.
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Authors | Ma Deqin, Zhao Chen, Christopher Nero, Keyur P Patel, Emad M Daoud, Hanyin Cheng, Bojana Djordjevic, Russell R Broaddus, L Jeffrey Medeiros, Asif Rashid, Rajyalakshmi Luthra |
Journal | Archives of pathology & laboratory medicine
(Arch Pathol Lab Med)
Vol. 136
Issue 5
Pg. 510-6
(May 2012)
ISSN: 1543-2165 [Electronic] United States |
PMID | 22540299
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Chemical References |
- 3' Untranslated Regions
- KRAS protein, human
- Proto-Oncogene Proteins
- Poly A
- BRAF protein, human
- Proto-Oncogene Proteins B-raf
- Proto-Oncogene Proteins p21(ras)
- ras Proteins
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Topics |
- 3' Untranslated Regions
(genetics)
- Base Sequence
- Colorectal Neoplasms
(genetics)
- Endometrial Neoplasms
(genetics)
- Female
- Genes, erbB-1
(genetics)
- Humans
- Microdissection
- Microsatellite Instability
- Molecular Sequence Data
- Mutation
- Poly A
(genetics)
- Proto-Oncogene Proteins
(genetics)
- Proto-Oncogene Proteins B-raf
(genetics)
- Proto-Oncogene Proteins p21(ras)
- Real-Time Polymerase Chain Reaction
- Sequence Deletion
- ras Proteins
(genetics)
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