Development of an immunoassay for the derived-peptide of chromogranin A, vasostatin-I (1-76): assessment of severity in patients with sepsis.

Abstract

CONTEXT:

Proteolytic fragments of chromogranin A (CgA) including the CgA 1-76 fragment (called vasostatin-I [VS-I]) could be a useful biomarker of sepsis, but there is no available immunoassay.

METHODS:

A sandwich ELISA for VS-I was developed, and plasma VS-I was measured in 30 healthy controls and 60 critically ill patients with sepsis.

RESULTS:

The ELISA showed intra- and inter-assay coefficients of variations (CVs) below 4 and 9%. Plasma VS-I was significantly increased compared with controls in patients with sepsis, severe sepsis, and sepsis shock (p < 0.0001). Receiver operating curve (ROC) analyses indicated that plasma VS-I was more sensitive and specific than plasma CgA to diagnose sepsis and to assess its severity.

CONCLUSIONS:

The measurements of plasma VS-I with this new ELISA may be useful for the clinical investigation of patients with sepsis.

• Authors: Hélène Chung, Angelo Corti, Luca Crippa, Francis Schneider, Marie-Hélène Metz-Boutigue, Patrick Garnero
• Journal: Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals (Biomarkers) Vol. 17 Issue 5 Pg. 430-4 (Aug 2012) ISSN: 1366-5804 [Electronic] England
• PMID: 22524436 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers
• Chromogranin A
• Peptide Fragments
• vasostatin I

Topics

• Adult
• Aged
• Biomarkers (blood)
• Chromogranin A (blood)
• Female
• Humans
• Immunoassay (methods)
• Male
• Middle Aged
• Peptide Fragments (blood)
• ROC Curve
• Reproducibility of Results
• Sepsis (blood, diagnosis)
• Severity of Illness Index
• Shock, Septic (blood, diagnosis)