Heparanase-induced GEF-H1 signaling regulates the cytoskeletal dynamics of brain metastatic breast cancer cells.

Abstract

Heparanase is the only mammalian endoglycosidase which has been widely implicated in cancer because of its capability to degrade heparan sulfate chains of heparan sulfate proteoglycans (HSPG). Specifically, the cell surface HSPG syndecan-1 and -4 (SDC1 and SDC4) are modulators of growth factor action, and SDC4 is implicated in cell adhesion as a key member of focal adhesion complexes. We hypothesized that extracellular heparanase modulates brain metastatic breast cancer (BMBC) cell invasiveness by affecting cytoskeletal dynamics, SDC4 carboxy-terminal-associated proteins, and downstream targets. We used two independently derived human BMBC cell systems (MB-231BR and MB-231BR3), which possess distinct cellular morphologies and properties. Highly aggressive spindle-shaped 231BR3 cells changed to a round cell morphology associated with expression of the small GTPase guanine nucleotide exchange factor-H1 (GEF-H1). We showed that GEF-H1 is a new component of the SDC4 signaling complex in BMBC cells. Treatment with heparanase resulted in regulation of the SDC4/protein kinase C α axis while maintaining a constitutive GEF-H1 level. Third, GEF-H1 knockdown followed by cell exposure to heparanase caused a significant regulation of activities of Rac1 and RhoA, which are GEF-H1 targets and fundamental effectors in cell plasticity control. Fourth, L-heparanase augmented expression of β1 integrin in BMBC cells and of vascular cell adhesion molecule 1 (VCAM1; the major β1 integrin receptor) in human brain microvascular endothelial cells. Finally, using a newly developed blood-brain barrier in vitro model, we show that BMBC cell transmigration was significantly reduced in GEF-H1 knockdown cells. These findings implicate heparanase in mechanisms of cytoskeletal dynamics and in the cross-talk between tumor cells and vascular brain endothelium. They are of relevance because they elucidate molecular events in the initial steps leading to BMBC onset and capturing distinct roles of latent and active heparanase in the brain microenvironment.

• Authors: Lon D Ridgway, Michael D Wetzel, Jason A Ngo, Anat Erdreich-Epstein, Dario Marchetti
• Journal: Molecular cancer research : MCR (Mol Cancer Res) Vol. 10 Issue 6 Pg. 689-702 (Jun 2012) ISSN: 1557-3125 [Electronic] United States
• PMID: 22513363 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
• Copyright: 2012 AACR

Chemical References

• ARHGEF2 protein, human
• Guanine Nucleotide Exchange Factors
• Integrin beta1
• Rho Guanine Nucleotide Exchange Factors
• SDC4 protein, human
• Syndecan-4
• Vascular Cell Adhesion Molecule-1
• heparanase
• Glucuronidase
• GTP Phosphohydrolases

Topics

• Blotting, Western
• Brain Neoplasms (genetics, metabolism, secondary)
• Breast Neoplasms (genetics, metabolism, pathology)
• Cell Adhesion (drug effects)
• Cell Line, Tumor
• Cell Movement (drug effects)
• Cell Shape (drug effects)
• Cytoskeleton (drug effects, metabolism)
• Endothelial Cells (metabolism)
• Female
• GTP Phosphohydrolases (metabolism)
• Gene Expression Regulation, Neoplastic (drug effects)
• Glucuronidase (pharmacology)
• Guanine Nucleotide Exchange Factors (genetics, metabolism)
• Humans
• Integrin beta1 (metabolism)
• Microscopy, Fluorescence
• Models, Biological
• Protein Binding (drug effects)
• RNA Interference
• Reverse Transcriptase Polymerase Chain Reaction
• Rho Guanine Nucleotide Exchange Factors
• Signal Transduction (genetics)
• Syndecan-4 (metabolism)
• Vascular Cell Adhesion Molecule-1 (metabolism)