Nanoparticle-based adjuvant for enhanced protective efficacy of DNA vaccine Ag85A-ESAT-6-IL-21 against Mycobacterium tuberculosis infection.

Abstract

The goal of this study was to evaluate the protective efficacy of a cationic nanoparticle-based DNA vaccine expressing antigen 85A (Ag85A) and 6-kDa early secretory antigen target (ESAT-6) of Mycobacterium tuberculosis as well as cytokine interleukin-21 (IL-21) against M. tuberculosis infection. The results of this indicated that the anti-M. tuberculosis immune responses were induced in mice that had received the different DNA vaccines. More importantly, compared with using DNA vaccine Ag85A-ESAT-6-IL-21 alone, the nanoparticle-based DNA vaccine Ag85A-ESAT-6-IL-21 showed a statistically significant increase in the protective efficacy against M. tuberculosis infection in the immunized mice. We concluded that the nanoparticle-based DNA vaccine induced a strong immune response and markedly inhibited the growth of the M. tuberculosis in the mice. These findings highlighted the potential utility of Fe3O4-Glu-polyethyleneimine nanoparticles encapsulated with the DNA vaccine as a prophylactic vaccine in the M. tuberculosis-infected mouse model.

FROM THE CLINICAL EDITOR:

This study emphasizes the potential utility of Fe3O4-Glu-polyethyleneimine nanoparticles encapsulated with DNA vaccine against TB as a prophylactic vaccine. The authors demonstrated a strong immune response and marked growth inhibition of mycobacterium tuberculosis in the mice.

• Authors: Fangliu Yu, Jing Wang, Jun Dou, Haitao Yang, Xingfeng He, Weiguo Xu, Yu Zhang, Kai Hu, Ning Gu
• Journal: Nanomedicine : nanotechnology, biology, and medicine (Nanomedicine) Vol. 8 Issue 8 Pg. 1337-44 (Nov 2012) ISSN: 1549-9642 [Electronic] United States
• PMID: 22406425 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 Elsevier Inc. All rights reserved.

Chemical References

• Adjuvants, Immunologic
• Ag85B-ESAT-6 fusion antigen
• Interleukins
• Recombinant Fusion Proteins
• Tuberculosis Vaccines
• Vaccines, DNA
• interleukin-21

Topics

• Adjuvants, Immunologic
• Animals
• Humans
• Interleukins (chemistry, immunology)
• Male
• Mice
• Mycobacterium tuberculosis (immunology, pathogenicity)
• Nanoparticles (chemistry)
• Recombinant Fusion Proteins (chemistry, immunology)
• Tuberculosis (immunology, microbiology, prevention & control)
• Tuberculosis Vaccines (administration & dosage, immunology)
• Vaccines, DNA (chemistry, immunology)