Protective effect of extract of Acanthopanax senticosus Harms on dopaminergic neurons in Parkinson's disease mice.

Abstract

To study the neuroprotective effect of extract of Acanthopanax senticosus Harms against MPTP-induced mice model of Parkinson's disease and its mechanism. The Parkinson's disease mice model was induced by 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine Hydrochloride (MPTP-HCl, 30mg/kg daily for 5 days). High dose group and low dose group were medicated with extract of Acanthopanax senticosus Harms for 20 days, dose amounted to 182mg/kg and 45.5mg/kg daily respectively. The behavioral testing of mice was assessed using pole-climbing test. The levels of Dopamine (DA) and Homovanillic acid (HVA) in striatum were determined by Ultra-performance liquid chromatography combined with time-of-flight mass spectrometry (UPLC-ToF-MS). The levels of dopamine receptor 1 and 2 in striatum were assayed simultaneously with the help of immunohistochemical method. The level of Caspase-3 protein in substantia nigra was analyzed by Western Blot. From Day 5 during the administration of extract of Acanthopanax senticosus Harms, pole-climbing time in low and high dose group were significantly less than model group (p<0.05). Compared with model group, the DA level of striatum in low dose group was significantly higher (p<0.01), the number of dopamine receptor 1 and dopamine receptor 2-positive cells in low and high dose group were significantly less (p<0.05), the Caspase-3 protein level of substantia nigra in low and high dose group were significantly less (p<0.05). The neuroprotective effect of extract of Acanthopanax senticosus Harms may be able to protect C57BL/6 mice against MPTP-induced dopaminergic neuronal damage.

• Authors: Shu-min Liu, Xu-zhao Li, Yan Huo, Fang Lu
• Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology (Phytomedicine) Vol. 19 Issue 7 Pg. 631-8 (May 15 2012) ISSN: 1618-095X [Electronic] Germany
• PMID: 22402244 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 Elsevier GmbH. All rights reserved.

Chemical References

• Neuroprotective Agents
• Plant Extracts
• Receptors, Dopamine
• 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
• Caspase 3
• Dopamine
• Homovanillic Acid

Topics

• 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
• Animals
• Behavior, Animal (drug effects)
• Brain (drug effects, metabolism)
• Caspase 3 (metabolism)
• Chromatography, High Pressure Liquid
• Disease Models, Animal
• Dopamine (metabolism)
• Dopaminergic Neurons (drug effects)
• Eleutherococcus
• Homovanillic Acid (metabolism)
• Male
• Mass Spectrometry
• Mice
• Mice, Inbred C57BL
• Neuroprotective Agents (pharmacology, therapeutic use)
• Parkinson Disease (drug therapy, etiology, metabolism)
• Phytotherapy
• Plant Extracts (pharmacology, therapeutic use)
• Receptors, Dopamine (metabolism)