Abstract | BACKGROUND: Mounting evidence shows that nuclear factor-κB (NF-κB) plays an important role in endometriosis. We therefore evaluated the therapeutic potential of andrographolide, an NF-κB inhibitor. METHODS: Primary cell cultures were performed using ectopic endometrial tissue specimens and their homologous eutopic endometrial specimens from 16 women with endometriosis, as well as control samples from 4 women without endometriosis. Andrographolide was evaluated for an effect on cell proliferation and cell cycle, DNA-binding activity of NF-κB and expression of cyclooxygenase-2 (COX-2) and tissue factor (TF). In a rat model of endometriosis, andrographolide treatment was evaluated for an effect on lesion size, hotplate response latency and expression of phosphorylated p50 and p65, COX-2 and nerve growth factor ( NGF) in ectopic endometrium. RESULTS:
Andrographolide dose dependently suppressed proliferation and cell cycle progression, attenuated DNA-binding activity of NF-κB in endometriotic stromal cells and inhibited COX-2 and TF expression. In the rat experiment, induced endometriosis resulted in reduced response latency. Andrographolide treatment significantly reduced lesion size in a dose-dependent manner and significantly increased response latency. Andrographolide treatment also significantly reduced immunoreactivity of COX-2, phosphorylated p50 and p65, and NGF in ectopic endometrium. CONCLUSIONS:
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Authors | Yu Zheng, Xishi Liu, Sun-Wei Guo |
Journal | Human reproduction (Oxford, England)
(Hum Reprod)
Vol. 27
Issue 5
Pg. 1300-13
(May 2012)
ISSN: 1460-2350 [Electronic] England |
PMID | 22402211
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Diterpenes
- NF-kappa B
- Neoplasm Proteins
- Nucleocytoplasmic Transport Proteins
- p65 oncofetal mRNA transport protein, rat
- andrographolide
- Nerve Growth Factor
- Cyclooxygenase 2
- ATPases Associated with Diverse Cellular Activities
- DNA Helicases
- Ruvbl1 protein, rat
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Topics |
- ATPases Associated with Diverse Cellular Activities
- Animals
- Cell Cycle
(drug effects)
- Cell Proliferation
(drug effects)
- Cells, Cultured
- Cyclooxygenase 2
(metabolism)
- DNA Helicases
(metabolism)
- Diterpenes
(pharmacology, therapeutic use)
- Endometriosis
(drug therapy, pathology)
- Female
- Humans
- NF-kappa B
(antagonists & inhibitors, metabolism)
- Neoplasm Proteins
(metabolism)
- Nerve Growth Factor
(metabolism)
- Nucleocytoplasmic Transport Proteins
(metabolism)
- Phosphorylation
- Rats
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