[Pathologic observation on animal model of silicosis].

Abstract

OBJECTIVE:

To explore the pathological changes of pulmonary fibrosis induced by SiO2 in rats and pigs.

METHODS:

The silicosis models in rats and pigs were established by non-exposure method. The pathologic changes in lung tissues of rats and pigs were observed with HE staining under a light microscopy and under a transmission electron microscopy (TEM), the expression of cytokines was detected by immunohistochemistry.

RESULTS:

(1) The main pathologic changes of silicosis models in rats and pigs included: in 7 ∼ 15 days after treatment, silica dusts, dust cells, a lot of macrophages, lung epithelial cells, a few neutrophils, macrophage alveolar inflammation and nodules of stage I were found in alveolar space; in 30 ∼ 90 days after treatment, many nodules of stage I-III or IV with lymphocytes infiltration were observed in respiratory bronchioles, alveoli, interlobular septa, the subpleural and around blood vessels and bronchi. (2) The expression levels of CK protein, SP-A protein, CD68, b-FGF, TNF-α, IL-6, TGF-β1, NFKappa/P50, Kappa/P65 and VEGF reduced with exposure time, but still were higher than those of the control. (3) The shed alveolar type I cells, proliferation of alveolar type II cells or macrophages and activated cellular function induced by silica were observed under TEM.

CONCLUSION:

The development of pulmonary fibrosis in silicosis models corresponded with the process from macrophages alveolar inflammation to pulmonary fibrosis.

• Authors: Xin-ming Pu, Hao Wen, Hong Dou, Zhi-xin Xu, Pei-chen Liu, Sai-jun Li, Ou Bai, Xin Wu, Han-xin Su, Xin-jun Liu, Rui-kang Jiang, Qu Zhang
• Journal: Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases (Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi) Vol. 29 Issue 10 Pg. 761-5 (Oct 2011) ISSN: 1001-9391 [Print] China
• PMID: 22357492 (Publication Type: English Abstract, Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Cytokines

Topics

• Animals
• Cytokines (metabolism)
• Disease Models, Animal
• Epithelial Cells (metabolism)
• Female
• Lung (cytology, pathology)
• Macrophages, Alveolar (metabolism)
• Male
• Neutrophils (metabolism)
• Rats
• Rats, Sprague-Dawley
• Silicosis (pathology)
• Swine