Contribution of IL-33-activated type II innate lymphoid cells to pulmonary eosinophilia in intestinal nematode-infected mice.

Abstract

When animals are infected with helminthic parasites, resistant hosts show type II helper T immune responses to expel worms. Recently, natural helper (NH) cells or nuocytes, newly identified type II innate lymphoid cells, are shown to express ST2 (IL-33 receptor) and produce IL-5 and IL-13 when stimulated with IL-33. Here we show the relevant roles of endogenous IL-33 for Strongyloides venezuelensis infection-induced lung eosinophilic inflammation by using Il33(-/-) mice. Alveolar epithelial type II cells (ATII) express IL-33 in their nucleus. Infection with S. venezuelensis or intranasal administration of chitin increases in the number of ATII cells and the level of IL-33. S. venezuelensis infection induces pulmonary accumulation of NH cells, which, after being stimulated with IL-33, proliferate and produce IL-5 and IL-13. Furthermore, S. venezuelensis infected Rag2(-/-) mice increase the number of ATII cells, NH cells, and eosinophils and the expression of IL-33 in their lungs. Finally, IL-33-stimulated NH cells induce lung eosinophilic inflammation and might aid to expel infected worms in the lungs.

• Authors: Koubun Yasuda, Taichiro Muto, Tatsukata Kawagoe, Makoto Matsumoto, Yuki Sasaki, Kazufumi Matsushita, Yuko Taki, Shizue Futatsugi-Yumikura, Hiroko Tsutsui, Ken J Ishii, Tomohiro Yoshimoto, Shizuo Akira, Kenji Nakanishi
• Journal: Proceedings of the National Academy of Sciences of the United States of America (Proc Natl Acad Sci U S A) Vol. 109 Issue 9 Pg. 3451-6 (Feb 28 2012) ISSN: 1091-6490 [Electronic] United States
• PMID: 22331917 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA-Binding Proteins
• Il33 protein, mouse
• Interleukin-13
• Interleukin-33
• Interleukin-5
• Interleukins
• Rag2 protein, mouse

Topics

• Animals
• Bronchoalveolar Lavage Fluid (cytology)
• CD4-Positive T-Lymphocytes (immunology)
• DNA-Binding Proteins (deficiency, genetics)
• Interleukin-13 (biosynthesis)
• Interleukin-33
• Interleukin-5 (biosynthesis)
• Interleukins (biosynthesis, deficiency, genetics, physiology)
• Intestinal Diseases, Parasitic (complications, immunology, pathology)
• Larva
• Lymphocytes (drug effects, immunology, metabolism)
• Mice
• Mice, Inbred C57BL
• Mice, Knockout
• Nippostrongylus (growth & development, immunology)
• Pulmonary Alveoli (immunology, pathology)
• Pulmonary Eosinophilia (etiology, immunology)
• Strongylida Infections (complications, immunology, pathology)
• Strongyloides (growth & development, immunology)
• Strongyloidiasis (complications, immunology, pathology)