Genome-wide analysis of HMGA2 transcription factor binding sites by ChIP on chip in gastric carcinoma cells.

Abstract

High mobility group protein A2 (HMGA2) is an architectural transcription factor that plays an important role in development and progression of malignant neoplasias. Recently, some studies reported that HMGA2 is also implicated in epithelial-mesenchymal transitions (EMT) and cancer stem cells. But the underlying mechanisms of these conditions are poorly understood. Therefore, we established an EMT model of gastric carcinoma cells by overexpressing HMGA2 in vitro, then global mapping of HMGA2 potential transcription factor binding sites was identified by promoter microarray in these cells, and the date obtained from the microarrays were validated via chromatin immunoprecipitation-PCR (ChIP-PCR) and real-time PCR. HMGA2 potential target genes were classified in KEGG database and Gene Ontology (GO) analyses. To our knowledge, this is the first report on the genome-wide analysis of HMGA2 downstream direct targets, and these findings will be valuable in understanding the roles of HMGA2 in EMT.

• Authors: Lang Zha, Ziwei Wang, Weixue Tang, Neng Zhang, Gang Liao, Zhen Huang
• Journal: Molecular and cellular biochemistry (Mol Cell Biochem) Vol. 364 Issue 1-2 Pg. 243-51 (May 2012) ISSN: 1573-4919 [Electronic] Netherlands
• PMID: 22246783 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• HMGA2 Protein
• Proteins

Topics

• Binding Sites
• Cell Line, Tumor
• Cell Movement (genetics)
• Cell Proliferation
• Databases, Genetic
• Epithelial-Mesenchymal Transition (genetics)
• Gene Expression Regulation, Neoplastic
• Genome, Human
• HMGA2 Protein (genetics, metabolism)
• Humans
• Neoplasm Invasiveness (genetics)
• Oligonucleotide Array Sequence Analysis
• Promoter Regions, Genetic
• Proteins (classification, genetics, metabolism)
• Signal Transduction (genetics)
• Stomach Neoplasms (genetics, metabolism, pathology)