CDP-choline treatment induces brain plasticity markers expression in experimental animal stroke.

Abstract

We investigated the effect of CDP-choline on brain plasticity markers expression in the acute phase of cerebral infarct in an experimental animal model. Male Sprague-Dawley rats were subjected to permanent middle cerebral artery occlusion (pMCAO) and treated or not with CDP-choline (500 mg/kg) daily for 14 days starting 30 min after pMCAO. Functional status was evaluated with Roger's test; lesion volume with magnetic resonance imaging (MRI) and hematoxylin and eosin staining (H&E); cell death with TUNEL; cellular proliferation with BrdU immunohistochemistry; vascular endothelial growth factor (VEGF), synaptophysin, glial fibrillary acidic protein (GFAP) and low-density lipoprotein receptor-related protein (LRP) by immunofluorescence and Western-blot techniques. CDP-choline significantly improved functional recovery and decreased lesion volume on MRI, TUNEL-positive cell number and LRP levels at 14 days. In addition, CDP-choline significantly increased BrdU, VEGF and synaptophysin values and decreased GFAP levels in the peri-infarct zone compared with the infarct group. In conclusion, our data indicate that CDP-choline improved functional recovery after permanent middle cerebral artery occlusion in association with reductions in lesion volume, cell death and LRP expression. In fact, CDP-choline increased cell proliferation, vasculogenesis and synaptophysin levels and reduced GFAP levels in the peri-infarct area of the ischemic stroke.

• Authors: María Gutiérrez-Fernández, Berta Rodríguez-Frutos, Blanca Fuentes, María Teresa Vallejo-Cremades, Julia Alvarez-Grech, Mercedes Expósito-Alcaide, Exuperio Díez-Tejedor
• Journal: Neurochemistry international (Neurochem Int) Vol. 60 Issue 3 Pg. 310-7 (Feb 2012) ISSN: 1872-9754 [Electronic] England
• PMID: 22226841 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 Elsevier Ltd. All rights reserved.

Chemical References

• Biomarkers
• Glial Fibrillary Acidic Protein
• Nootropic Agents
• Synaptophysin
• Vascular Endothelial Growth Factor A
• Cytidine Diphosphate Choline

Topics

• Animals
• Apoptosis (drug effects)
• Biomarkers
• Blotting, Western
• Brain Chemistry (drug effects)
• Brain Ischemia (complications, pathology)
• Cell Proliferation (drug effects)
• Cytidine Diphosphate Choline (pharmacology)
• Glial Fibrillary Acidic Protein (biosynthesis, genetics)
• Immunohistochemistry
• In Situ Nick-End Labeling
• Infarction, Middle Cerebral Artery (complications, pathology)
• Magnetic Resonance Imaging
• Male
• Neuronal Plasticity (drug effects)
• Nootropic Agents (pharmacology)
• Rats
• Rats, Sprague-Dawley
• Stroke (drug therapy, metabolism, pathology)
• Synaptophysin (biosynthesis, genetics)
• Vascular Endothelial Growth Factor A (biosynthesis, genetics)