Effects of hypoxia on osteogenic differentiation of rat bone marrow mesenchymal stem cells.

Abstract

Bone reconstruction is essential in orthodontic treatment that caters to the correction of malocclusion by bone reconstruction. Mesenchymal stem cells (MSCs) have been demonstrated a great potency of osteogenesis. The aim of this study was to investigate the effect of hypoxia on the rat bone marrow MSCs (rBMSCs) in vitro during osteogenesis. In this study, we found that temporary exposure of rBMSCs after osteogenic induction for 7 days to hypoxia (2% oxygen) led to a marked decrease in ALPase activity and the expression of osteocalcin and Runt related transcription factor 2/core binding factor a1 (Runx2/Cbfa1). Meanwhile, we found that exposure to hypoxia led to an early and transient increase in the level of phosphorylated ERK1/2 but had no obvious effects on mitogen-activated protein kinase (p38 MAPK) level. Based on these results, we concluded that hypoxia could inhibit osteogenic differentiation of rBMSCs possibly through MEK-ERK 1/2, while p38 MAPK may not participate in this regulation. Further exploration into the mechanisms of hypoxia on osteogenesis would surely provide reliable evidence for clinical practice.

• Authors: Yating Wang, Juan Li, Yanmin Wang, Lei Lei, Chunmiao Jiang, Shu An, Yuxiang Zhan, Qian Cheng, Zhihe Zhao, Jun Wang, Lingyong Jiang
• Journal: Molecular and cellular biochemistry (Mol Cell Biochem) Vol. 362 Issue 1-2 Pg. 25-33 (Mar 2012) ISSN: 1573-4919 [Electronic] Netherlands
• PMID: 22198287 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Core Binding Factor Alpha 1 Subunit
• RNA, Messenger
• Runx2 protein, rat
• Osteocalcin
• Mitogen-Activated Protein Kinase 1
• Mitogen-Activated Protein Kinase 3
• p38 Mitogen-Activated Protein Kinases
• Alkaline Phosphatase

Topics

• Alkaline Phosphatase (genetics, metabolism)
• Animals
• Bone Marrow Cells
• Bone Remodeling
• Cell Differentiation (physiology)
• Cell Hypoxia
• Core Binding Factor Alpha 1 Subunit (biosynthesis, genetics)
• MAP Kinase Signaling System
• Mesenchymal Stem Cells (cytology, physiology)
• Mitogen-Activated Protein Kinase 1 (metabolism)
• Mitogen-Activated Protein Kinase 3 (metabolism)
• Osteocalcin (biosynthesis, genetics)
• Osteogenesis (physiology)
• RNA, Messenger (genetics, metabolism)
• Rats
• p38 Mitogen-Activated Protein Kinases (metabolism)