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Development of a highly sensitive immunochromatographic detection kit for H5 influenza virus hemagglutinin using silver amplification.

Abstract
H5N1, a highly pathogenic avian influenza virus (HPAIV), has become a serious epizootic threat to the poultry population in Asia. In addition, significant numbers of human cases of HPAIV infection have been reported to date. To prevent the spread of HPAIV among humans and to allow for timely medical intervention, a rapid and high sensitive method is needed to detect and subtype the causative HPAIVs. In the present study, a silver amplification technique used in photographic development was combined with immunochromatography technologies and a highly sensitive and rapid diagnostic test to detect the hemagglutinin of H5 influenza viruses was developed. The sensitivity of the test kit was increased 500 times by silver amplification. The sensitivity of the method was more than 10 times higher than those of conventional rapid influenza diagnostic tests, which detect viral nucleoproteins. The diagnostic system developed in the present study can therefore provide rapid and highly sensitive results and will be useful for diagnosis of H5 HPAIV infection in humans and animals.
AuthorsAtsuhiko Wada, Yoshihiro Sakoda, Takayoshi Oyamada, Hiroshi Kida
JournalJournal of virological methods (J Virol Methods) Vol. 178 Issue 1-2 Pg. 82-6 (Dec 2011) ISSN: 1879-0984 [Electronic] Netherlands
PMID21911008 (Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2011 Elsevier B.V. All rights reserved.
Chemical References
  • Hemagglutinin Glycoproteins, Influenza Virus
  • hemagglutinin, avian influenza A virus
  • Silver
Topics
  • Animals
  • Chromatography (methods)
  • Clinical Laboratory Techniques (methods)
  • Hemagglutinin Glycoproteins, Influenza Virus (analysis)
  • Humans
  • Immunoassay (methods)
  • Influenza A Virus, H5N1 Subtype (chemistry, isolation & purification)
  • Influenza in Birds (diagnosis, virology)
  • Influenza, Human (diagnosis, virology)
  • Poultry
  • Sensitivity and Specificity
  • Silver (metabolism)
  • Staining and Labeling (methods)

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