Downregulation of GRIM-19 promotes growth and migration of human glioma cells.

Abstract

It has become increasingly clear that there are notable parallels between normal development and tumorigenesis. Glioma is a classic model that links between tumorigenesis and development. We evaluated the expression of GRIM-19, a novel gene essential for normal development, in various grades of gliomas and several human glioma cell lines. We showed that GRIM-19 mRNA and protein expression were markedly lower in gliomas than in control brain tissues and negatively correlated with the malignancy of gliomas. Downregulation of GRIM-19 in glioma cells significantly enhanced cell proliferation and migration, whereas overexpression of GRIM-19 showed the opposite effects. We also showed that the activation of signal transducer and activator of transcription 3 (STAT3) and the expression of many STAT3-dependent genes were regulated by the expression of GRIM-19. In addition, GRIM-19 exerted its role probably through the non-STAT3 signaling pathway. Collectively, our data suggest that most gliomas expressed GRIM-19 at low levels, which may play a major role in tumorigenesis in the brain.

• Authors: Yanmin Zhang, Hongbo Hao, Shidou Zhao, Qian Liu, Qiuhuan Yuan, Shilei Ni, Fuwu Wang, Shangming Liu, Liyan Wang, Aijun Hao
• Journal: Cancer science (Cancer Sci) Vol. 102 Issue 11 Pg. 1991-9 (Nov 2011) ISSN: 1349-7006 [Electronic] England
• PMID: 21827581 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2011 Japanese Cancer Association.

Chemical References

• Apoptosis Regulatory Proteins
• DDIT3 protein, human
• Neoplasm Proteins
• Nerve Tissue Proteins
• Recombinant Fusion Proteins
• STAT3 Transcription Factor
• STAT3 protein, human
• Transcription Factor CHOP
• Cyclooxygenase 2
• PTGS2 protein, human
• NADH, NADPH Oxidoreductases
• NDUFA13 protein, human

Topics

• Apoptosis
• Apoptosis Regulatory Proteins (biosynthesis, genetics, physiology)
• Brain (metabolism)
• Brain Neoplasms (genetics, metabolism, pathology)
• Cell Division (physiology)
• Cell Movement (physiology)
• Cyclooxygenase 2
• Gene Expression Regulation, Neoplastic
• Glioblastoma (metabolism, pathology)
• Glioma (genetics, metabolism, pathology)
• Humans
• NADH, NADPH Oxidoreductases (biosynthesis, genetics, physiology)
• Neoplasm Proteins (biosynthesis, genetics, physiology)
• Nerve Tissue Proteins (biosynthesis, genetics, physiology)
• Phosphorylation
• Protein Processing, Post-Translational
• RNA Interference
• Recombinant Fusion Proteins (physiology)
• STAT3 Transcription Factor (physiology)
• Transcription Factor CHOP
• Tumor Cells, Cultured (pathology)
• Tumor Stem Cell Assay