The glutaredoxin ATGRXS13 is required to facilitate Botrytis cinerea infection of Arabidopsis thaliana plants.

Abstract

Botrytis cinerea is a major pre- and post-harvest necrotrophic pathogen with a broad host range that causes substantial crop losses. The plant hormone jasmonic acid (JA) is involved in the basal resistance against this fungus. Despite basal resistance, virulent strains of B. cinerea can cause disease on Arabidopsis thaliana and virulent pathogens can interfere with the metabolism of the host in a way to facilitate infection of the plant. However, plant genes that are required by the pathogen for infection remain poorly described. To find such genes, we have compared the changes in gene expression induced in A. thaliana by JA with those induced after B. cinerea using genome-wide microarrays. We have identified genes that are repressed by JA but that are induced by B. cinerea. In this study, we describe one candidate gene, ATGRXS13, that encodes for a putative glutaredoxin and that exhibits such a crossed expression. In plants that are infected by this necrotrophic fungus, ATGRXS13 expression was negatively controlled by JA and TGA transcription factors but also through a JA-salicylic acid (SA) cross-talk mechanism as B. cinerea induced SA production that positively controlled ATGRXS13 expression. Furthermore, plants impaired in ATGRXS13 exhibited resistance to B. cinerea. Finally, we present a model whereby B. cinerea takes advantage of defence signalling pathways of the plant to help the colonization of its host.

• Authors: Sylvain La Camera, Floriane L'haridon, Jérémy Astier, Mark Zander, Eliane Abou-Mansour, Gonzague Page, Corinna Thurow, David Wendehenne, Christiane Gatz, Jean-Pierre Métraux, Olivier Lamotte
• Journal: The Plant journal : for cell and molecular biology (Plant J) Vol. 68 Issue 3 Pg. 507-19 (Nov 2011) ISSN: 1365-313X [Electronic] England
• PMID: 21756272 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2011 The Authors. The Plant Journal © 2011 Blackwell Publishing Ltd.

Chemical References

• Arabidopsis Proteins
• Cyclopentanes
• Glutaredoxins
• Oxylipins
• RNA, Plant
• Transcription Factors
• jasmonic acid
• Salicylic Acid

Topics

• Alternative Splicing
• Amino Acid Sequence
• Arabidopsis (enzymology, genetics, microbiology)
• Arabidopsis Proteins (genetics, metabolism)
• Botrytis (pathogenicity)
• Cloning, Molecular
• Cyclopentanes (metabolism)
• Disease Resistance
• Gene Expression Regulation, Plant
• Glutaredoxins (genetics, metabolism)
• Molecular Sequence Data
• Mutagenesis, Insertional
• Oligonucleotide Array Sequence Analysis
• Oxylipins (metabolism)
• Plant Diseases (genetics, microbiology)
• RNA, Plant (genetics)
• Salicylic Acid (metabolism)
• Signal Transduction
• Transcription Factors (metabolism)