Neutrophils are the most abundant circulating FcR-expressing WBCs with potent cytotoxic ability. Currently, they are recognized as promising effector cells for Ab-mediated
immunotherapy of
cancer, because their capacity to kill
tumor cells is greatly enhanced by
tumor Ag-specific mAbs. The FcαRI represents the most potent FcR on neutrophils for induction of Ab-mediated
tumor cell killing. However, the mechanisms of cell death that are induced are poorly understood. Because these mechanisms can be used for modulation of anticancer treatment, we investigated the
tumor cell death induced by neutrophil-mediated Ab-dependent killing via FcαRI.
Human mammary carcinoma cells were efficiently killed when incubated with human neutrophils and
tumor-specific FcαRI bispecific or
IgA Abs. Interestingly, we observed characteristics of autophagy such as autophagic structures by electron microscopy and LC3B(+) autophagosomes in different human epithelial
carcinoma cells, which resulted in
tumor cell death. To a lesser extent, necrotic features, such as cellular membrane breakdown and spillage of intracellular content, were found. By contrast, apoptotic features including fragmented nuclei,
Annexin V-positivity, and presence of cleaved
caspase-3 were not observed. These findings indicate that neutrophils mainly facilitate autophagy to induce
tumor cell death rather than the more commonly recognized apoptotic cell death mechanisms induced by NK cells or cytotoxic T cells. This knowledge not only reveals the type of
tumor cell death induced in neutrophil-mediated, Ab-dependent cellular cytotoxicity, but importantly opens up additional perspectives for modulation of anticancer
therapy in, for example, apoptosis-resistant
tumor cells.