ETS-1 protooncogene is an important
transcription factor that plays a role in the regulation of physiological processes, such as cell proliferation and differentiation. ETS-1 is thought to be related to the growth of
carcinoma cells by its regulation of the transcription of
matrix metalloproteinases and
urokinase-type plasminogen activator. In this study, we aimed to investigate the expression pattern of ETS-1
oncoprotein in urothelial
carcinomas of the urinary bladder and determine its relationship with histopathologic parameters, including
tumor grade and stage. One hundred six specimens of urothelial
carcinoma and a total of 14 normal urothelium were analyzed immunohistochemically with anti-ETS-1
monoclonal antibody. The normal urothelium showed positive ETS-1 immunostaining. ETS-1 expression remained high in low-grade and noninvasive
tumors, whereas it frequently decreased in high-grade or invasive
carcinomas. Interestingly, ETS-1 was highly expressed in the basal cell layer of the noninvasive urothelial
carcinomas. ETS-1 expression showed a strong negative correlation with the
tumor grade (P<0.001; r, -0.67) and stage (P<0.001; r, -0.75). The nonmuscle-invasive
tumors (pTa+pT1) and noninvasive
tumors (pTa) had significantly higher ETS-1 expression than the muscle-invasive
tumors (pT2; P<0.001) and invasive
tumors (pT1+pT2; P<0.001), respectively. Results of our study show that decreased ETS-1 expression is significantly associated with high grade and advanced stage in urothelial
carcinomas of the urinary bladder, and that the downregulation of ETS-1 expression may be a marker of the aggressiveness of such
malignancies.