High-performance liquid chromatography with photodiode array detection for determination of nobiletin content in the brain and serum of mice administrated the natural compound.

Abstract

We recently demonstrated that nobiletin, a citrus flavonoid, exhibits anti-dementia action in animals. However, no determination methods for the content of nobiletin with beneficial action in the brain of nobiletin-administered animals have been developed, nor has its pharmacokinetics been revealed completely. Here, we established the high-performance liquid chromatography/photodiode array detection method for nobiletin determination using Bond Elut C18 SPE cartridges for extraction, where the calibration curve was linear over 0.025-10 ng, with coefficient of variation of less than 6.76%. This method enabled us to determine pharmacokinetic parameters of nobiletin given intraperitoneally or per os in the brain of mice.

• Authors: Daisuke Saigusa, Masatoshi Shibuya, Daisuke Jinno, Hiroyuki Yamakoshi, Yoshiharu Iwabuchi, Akihito Yokosuka, Yoshihiro Mimaki, Akira Naganuma, Yasushi Ohizumi, Yoshihisa Tomioka, Tohru Yamakuni
• Journal: Analytical and bioanalytical chemistry (Anal Bioanal Chem) Vol. 400 Issue 10 Pg. 3635-41 (Jul 2011) ISSN: 1618-2650 [Electronic] Germany
• PMID: 21559759 (Publication Type: Journal Article)

Chemical References

• Antioxidants
• Flavones
• Flavonoids
• nobiletin

Topics

• Animals
• Antioxidants
• Brain (metabolism)
• Calibration
• Chromatography, High Pressure Liquid (instrumentation, methods, standards)
• Flavones (administration & dosage, blood, pharmacokinetics)
• Flavonoids
• Mice
• Reproducibility of Results