Apoptosis photoinduction by C60 fullerene in human leukemic T cells.

Abstract

The viability of normal (Wistar rat thymocytes) and transformed (human leukemia Jurkat cells) T cells after UV/Vis irradiation in the presence of pristine C60 fullerene was studied. The data obtained have shown that C60 fullerene exhibits cytotoxic effect against transformed T lymphocytes when combined with UV/Vis irradiation using mercury-vapor lamp (320-600 nm). C60 fullerene photocytotoxicity was not detected in thymocytes. C60-dependent photoinduced apoptosis of Jurkat cells was confirmed by DNA fragmentation and caspase-3 activation. No substantial increase of caspase-3 activation was observed in thymocytes treated with C60 fullerene plus irradiation, while antileukemic agent cytosine arabinoside was shown to induce caspase-3 activation both in Jurkat cells and thymocytes. The data obtained may be useful for development of photosensitizers for photodynamic therapy with selective action on leukemia cells.

• Authors: K O Palyvoda, I I Grynyuk, S V Prylutska, A A Samoylenko, L B Drobot, O P Matyshevska
• Journal: Ukrains'kyi biokhimichnyi zhurnal (1999 ) (Ukr Biokhim Zh (1999)) 2010 Jul-Aug Vol. 82 Issue 4 Pg. 121-7 Ukraine
• PMID: 21513214 (Publication Type: Journal Article)

Chemical References

• Fullerenes
• Photosensitizing Agents
• Caspase 3
• fullerene C60

Topics

• Animals
• Apoptosis (drug effects, radiation effects)
• Blotting, Western
• Caspase 3 (metabolism)
• Cell Survival (drug effects, radiation effects)
• DNA Fragmentation (drug effects, radiation effects)
• Female
• Fullerenes (pharmacology)
• Humans
• Jurkat Cells
• Light
• Photochemotherapy
• Photosensitizing Agents (pharmacology)
• Rats
• Rats, Wistar
• T-Lymphocytes (drug effects, pathology, radiation effects)
• Thymus Gland (cytology, drug effects, radiation effects)
• Time Factors