Chronic exposure to arsenite induces S100A8 and S100A9 expression in rat RBL-2H3 mast cells.

Abstract

To investigate the effects of chronic exposure to arsenite on the gene expression profiles of mast cells, microarray analysis was performed on rat basophilic leukemia RBL-2H3 cells exposed to arsenite for 28 days. Upregulated genes include calcium-binding S100 proteins such as S100A9, S100A10, S100A6, and S100A13, and granzymes B and C. Among S100 proteins, S100A9 showed the highest expression (8.62-fold of untreated cells) after 4-weeks of exposure to arsenite. As S100A8 and S100A9 comprise a heterodimer called calprotectin, and are implicated in the development of atherosclerosis and cancer, mRNA levels of both S100A8 and S100A9 were analyzed. The results demonstrated that exposure of RBL-2H3 cells to arsenite for a few weeks induces marked increases in mRNA levels of S100A8 and S100A9.

• Authors: Yuri Shimizu, Hitomi Fujishiro, Kanako Matsumoto, Daigo Sumi, Masahiko Satoh, Seiichiro Himeno
• Journal: The Journal of toxicological sciences (J Toxicol Sci) Vol. 36 Issue 1 Pg. 135-9 (Jan 2011) ISSN: 1880-3989 [Electronic] Japan
• PMID: 21297353 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Arsenites
• Calgranulin A
• Calgranulin B
• RNA, Messenger
• Sodium Compounds
• Water Pollutants, Chemical
• sodium arsenite

Topics

• Animals
• Arsenites (toxicity)
• Atherosclerosis (etiology)
• Calgranulin A (genetics)
• Calgranulin B (genetics)
• Gene Expression Profiling
• Leukemia, Basophilic, Acute
• Mast Cells (drug effects, metabolism)
• Microarray Analysis
• Neoplasms (etiology)
• RNA, Messenger (metabolism)
• Rats
• Sodium Compounds (toxicity)
• Time Factors
• Tumor Cells, Cultured
• Up-Regulation
• Water Pollutants, Chemical (toxicity)