Abstract | OBJECTIVE: RESEARCH DESIGN AND METHODS: Male C57BL/6 mice randomized to a high-fat diet or control diet were killed at 4-week intervals. DIO mice underwent switch to ad libitum low-fat diet (DIO-switch) or caloric restriction (CR) for 4 weeks before being killed. PyyKO mice and their DIO wild-type (WT) littermates underwent GIBP or sham surgery and were culled 10 days postoperatively. Fasting acyl-ghrelin, total PYY, active GLP-1 concentrations, stomach ghrelin expression, and colonic Pyy and glucagon expression were determined. Fasting and postprandial PYY and GLP-1 concentrations were assessed 30 days postsurgery in GIBP and sham pair-fed ( sham.PF) groups. RESULTS: DIO progressively reduced circulating fasting acyl-ghrelin, PYY, and GLP-1 levels. CR and DIO-switch caused weight loss but failed to restore circulating PYY to weight-appropriate levels. After GIBP, WT mice lost weight and exhibited increased circulating fasting PYY and colonic Pyy and glucagon expression. In contrast, the acute effects of GIBP on body weight were lost in PyyKO mice. Fasting PYY and postprandial PYY and GLP-1 levels were increased in GIBP mice compared with sham.PF mice. CONCLUSIONS: PYY plays a key role in mediating the early weight loss observed post-GIBP, whereas relative PYY deficiency during dieting may compromise weight-loss attempts.
|
Authors | Keval Chandarana, Cigdem Gelegen, Efthimia Karra, Agharul I Choudhury, Megan E Drew, Veronique Fauveau, Benoit Viollet, Fabrizio Andreelli, Dominic J Withers, Rachel L Batterham |
Journal | Diabetes
(Diabetes)
Vol. 60
Issue 3
Pg. 810-8
(Mar 2011)
ISSN: 1939-327X [Electronic] United States |
PMID | 21292870
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Ghrelin
- Leptin
- Peptide YY
- Glucagon-Like Peptide 1
- Glucagon
|
Topics |
- Analysis of Variance
- Animals
- Colon
(metabolism)
- Diet, Fat-Restricted
- Diet, Reducing
- Enzyme-Linked Immunosorbent Assay
- Gastric Bypass
- Gastric Mucosa
(metabolism)
- Ghrelin
(metabolism)
- Glucagon
(metabolism)
- Glucagon-Like Peptide 1
(metabolism)
- Leptin
(blood)
- Male
- Mice
- Obesity
(metabolism, surgery)
- Peptide YY
(metabolism)
- Radioimmunoassay
- Random Allocation
- Reverse Transcriptase Polymerase Chain Reaction
- Weight Loss
(physiology)
|