Effect of chronic ingestion of a fermented dairy product containing Lactobacillus acidophilus and Bifidobacterium bifidum on metabolic activities of the colonic flora in humans.

Abstract

Nine healthy volunteers were studied before, during, and after ingesting a fermented dairy product containing Lactobacillus acidophilus, Bifidobacterium bifidum, and mesophilic cultures (Streptococcus lactis and S cremoris) for 3 wk. Hydrogen and methane productions and fecal beta-galactosidase and beta-glucosidase activities were measured as indicators of fermentation capacity of the colonic flora. Fecal concentrations of nitroreductase, azoreductase, and beta-glucuronidase, which may be implicated in colonic carcinogenesis, were also assessed. Hydrogen and methane productions, fecal beta-galactosidase, beta-glucuronidase, and azoreductase activities did not change over three 3-wk periods whereas fecal beta-glucosidase activity increased (42 +/- 6, 91 +/- 12, and 40 +/- 6 IU/g N, P less than 0.01) and nitroreductase decreased (0.87 +/- 0.13, 0.54 +/- 0.11, and 0.57 +/- 0.08 IU/g N, P less than 0.05).

• Authors: P Marteau, P Pochart, B Flourié, P Pellier, L Santos, J F Desjeux, J C Rambaud
• Journal: The American journal of clinical nutrition (Am J Clin Nutr) Vol. 52 Issue 4 Pg. 685-8 (Oct 1990) ISSN: 0002-9165 [Print] United States
• PMID: 2119557 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Hydrogen
• NADH, NADPH Oxidoreductases
• Nitroreductases
• azoreductase
• beta-Glucosidase
• Glucuronidase
• Methane

Topics

• Adult
• Bifidobacterium (enzymology, physiology)
• Colon (microbiology)
• Dairy Products
• Feces (enzymology)
• Female
• Fermentation
• Glucuronidase (metabolism)
• Humans
• Hydrogen
• Lactobacillus acidophilus (physiology)
• Male
• Methane
• NADH, NADPH Oxidoreductases (metabolism)
• Nitroreductases
• Respiration
• Time Factors
• beta-Glucosidase (metabolism)