Detection of β-catenin, gastrokine-2 and embryonic stem cell expressed ras in gastric cancers.

Abstract

UNLABELLED:

ERas activation and GKN2 reduction in gastric cancer has raised some notices in recent years, while nuclear beta-catenin positivity is considered as a tumoral marker. In this study, we compared immunohistochemistry of beta-catenin, GKN2 and ERas on tumoral and non-tumoral mucosae of 50 gastric carcinomas and 13 gastric samples of cancer-free patients. Nuclear positivity of beta-catenin was strong in 31 non-tumoral mucosae (62%) and 29 tumoral mucosae (58%). It was absent in samples of cancer-free patients. There was a correlation between non-tumoral and tumoral zones for nuclear beta-catenin positivity (P=0.013). ERas was positive in 35 non-tumoral tissues (70%) and 31 tumoral tissues (62%) but negatvie in samples of cancer-free patients. It was weak and spotty in non-tumoral mucosae but strong and diffuse in tumors. Positivity of ERas was age-related (P=0.028). However it had background staining effect. GKN2 was expressed in 33 non-tumoral mucosae (66%) and 35 tumoral mucosae (70%). Though GKN2 staining was moderate to strong in non-tumoral tissues and was comparatively weaker in tumors, their difference was minimal and difficult to discern.

CONCLUSIONS:

Beta-catenin nuclear location could be considered as a paraneoplastic pattern which is considerably tumor-related. ERas may be a potential biomarker for gastric cancer, but advanced studies are wanted. GKN2 reduction is indiscernible by immunostaining.

• Authors: Fan Zhang, Jian Min Tang, Li Wang, Jing Ying Shen, Lin Zheng, Ping Ping Wu, Mei Zhang, Zhao Wen Yan
• Journal: International journal of clinical and experimental pathology (Int J Clin Exp Pathol) Vol. 3 Issue 8 Pg. 782-91 (Nov 01 2010) ISSN: 1936-2625 [Electronic] United States
• PMID: 21151392 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers, Tumor
• CTNNB1 protein, human
• Carrier Proteins
• ERas protein, human
• GKN2 protein, human
• beta Catenin
• Oncogene Protein p21(ras)

Topics

• Adenocarcinoma (metabolism, pathology)
• Adult
• Aged
• Aged, 80 and over
• Biomarkers, Tumor (metabolism)
• Carrier Proteins (metabolism)
• Cell Nucleus (metabolism, pathology)
• Embryonic Stem Cells (metabolism, pathology)
• Female
• Gastric Mucosa (metabolism, pathology)
• Humans
• Lymph Nodes (pathology)
• Male
• Middle Aged
• Oncogene Protein p21(ras) (metabolism)
• Stomach Neoplasms (metabolism, pathology)
• Tissue Array Analysis
• beta Catenin (metabolism)