Reduced expression of β2 integrin genes in rat peripheral leukocytes by inhibiting postprandial hyperglycemia.

Abstract

β(2) integrins (CD11s/CD18) promote the attachment of leukocytes to vascular endothelial cells. We performed in this study sucrose loading to rats with moderate postprandial hyperglycemia with/without once-daily dosing of the α-glucosidase inhibitor, miglitol, for 4 days under 4-h fasting conditions. The streptozotocin (STZ)-treated rats showed moderate postprandial hyperglycemia on days 1 and 4. The gene expression was higher for CD11a with fasting and 3-h postprandial on day 1, and with fasting on day 4, for CD11b with fasting on day 1 and 3-h postprandial on day 4, and for CD18 with fasting on days 1 and 4 in peripheral leukocytes from the STZ-treated rats than in peripheral leukocytes from the saline-treated rats. Miglitol reduced postprandial hyperglycemia and the gene expression of CD11a with fasting and of CD11b 3-h postprandial on day 4. These results indicate that inhibiting postprandial hyperglycemia reduced the mRNA expression of β(2) integrins in peripheral leukocytes of moderately postprandial hyperglycemic rats.

• Authors: Kazuki Mochizuki, Masaya Shimada, Yutaro Tanaka, Nanae Fukaya, Toshinao Goda
• Journal: Bioscience, biotechnology, and biochemistry (Biosci Biotechnol Biochem) Vol. 74 Issue 12 Pg. 2470-4 ( 2010) ISSN: 1347-6947 [Electronic] England
• PMID: 21150098 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Blood Glucose
• CD18 Antigens
• Enzyme Inhibitors
• Glycoside Hydrolase Inhibitors
• miglitol
• 1-Deoxynojirimycin
• Disaccharidases

Topics

• 1-Deoxynojirimycin (analogs & derivatives, pharmacology, therapeutic use)
• Animals
• Blood Glucose (metabolism)
• CD18 Antigens (genetics)
• Disaccharidases (antagonists & inhibitors)
• Enzyme Inhibitors (pharmacology, therapeutic use)
• Gene Expression Regulation (drug effects)
• Glycoside Hydrolase Inhibitors
• Hyperglycemia (blood, drug therapy, genetics)
• Intestine, Small (cytology)
• Leukocytes (drug effects, metabolism)
• Male
• Microvilli (enzymology)
• Rats
• Rats, Sprague-Dawley