Abstract | BACKGROUND: The molecular factors that control parathyroid tumorigenesis are poorly understood. In the absence of local invasion or metastasis, distinguishing benign from malignant parathyroid neoplasm is difficult on histologic examination. We studied the microRNA ( miRNA) profile in normal, hyperplastic, and benign and malignant parathyroid tumors to better understand the molecular factors that may play a role in parathyroid tumorigenesis and that may serve as diagnostic markers for parathyroid carcinoma. METHODS:
miRNA arrays containing 825 human microRNAs with four duplicate probes per miRNA were used to profile parathyroid tumor (12 adenomas, 9 carcinomas, and 15 hyperplastic) samples normalized to four reference normal parathyroid glands. Differentially expressed miRNA were validated by real-time quantitative TaqMan polymerase chain reaction (PCR). RESULTS: CONCLUSIONS:
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Authors | Reza Rahbari, Alisha K Holloway, Mei He, Elham Khanafshar, Orlo H Clark, Electron Kebebew |
Journal | Annals of surgical oncology
(Ann Surg Oncol)
Vol. 18
Issue 4
Pg. 1158-65
(Apr 2011)
ISSN: 1534-4681 [Electronic] United States |
PMID | 21086055
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers, Tumor
- MicroRNAs
- RNA, Messenger
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Topics |
- Adenoma
(genetics, metabolism)
- Biomarkers, Tumor
(genetics, metabolism)
- Gene Expression Profiling
- Gene Expression Regulation, Neoplastic
- Humans
- Hyperplasia
(metabolism, pathology)
- MicroRNAs
(genetics)
- Oligonucleotide Array Sequence Analysis
- Parathyroid Glands
(metabolism, pathology)
- Parathyroid Neoplasms
(genetics, metabolism)
- RNA, Messenger
(genetics)
- ROC Curve
- Reverse Transcriptase Polymerase Chain Reaction
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