Genome-wide study of familial juvenile hyperuricaemic (gouty) nephropathy (FJHN) indicates a new locus, FJHN3, linked to chromosome 2p22.1-p21.

Abstract

Familial juvenile hyperuricaemic (gouty) nephropathy (FJHN), is an autosomal dominant disease associated with a reduced fractional excretion of urate, and progressive renal failure. FJHN is genetically heterogeneous and due to mutations of three genes: uromodulin (UMOD), renin (REN) and hepatocyte nuclear factor-1beta (HNF-1β) on chromosomes 16p12, 1q32.1, and 17q12, respectively. However, UMOD, REN or HNF-1β mutations are found in only approximately 45% of FJHN probands, indicating the involvement of other genetic loci in approximately 55% of probands. To identify other FJHN loci, we performed a single nucleotide polymorphism (SNP)-based genome-wide linkage analysis, in six FJHN families in whom UMOD, HNF-1β and REN mutations had been excluded. Parametric linkage analysis using a 'rare dominant' model established linkage in five of the six FJHN families, with a LOD score >+3, at 0% recombination, between FJHN and SNPs at chromosome 2p22.1-p21. Analysis of individual recombinants in two unrelated affected individuals defined a approximately 5.5 Mbp interval, flanked telomerically by SNP RS372139 and centromerically by RS896986 that contained the locus, designated FJHN3. The interval contains 28 genes, and DNA sequence analysis of the most likely candidate, solute carrier family 8 member 1 (SLC8A1), did not identify any abnormalities in the FJHN3 probands. FJHN3 is likely located within a approximately 5.5 Mbp interval on chromosome 2p22.1-p21, and identifying the genetic abnormality will help to further elucidate mechanisms predisposing to gout and renal failure.

• Authors: Sian E Piret, Patrick Danoy, Karin Dahan, Anita A C Reed, Karena Pryce, William Wong, Rosa J Torres, Juan G Puig, Thomas Müller, Peter Kotanko, Karl Lhotta, Olivier Devuyst, Matthew A Brown, Rajesh V Thakker
• Journal: Human genetics (Hum Genet) Vol. 129 Issue 1 Pg. 51-8 (Jan 2011) ISSN: 1432-1203 [Electronic] Germany
• PMID: 20976470 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Uric Acid

Topics

• Chromosomes, Human, Pair 2 (genetics)
• Female
• Genetic Heterogeneity
• Genetic Linkage
• Genetic Loci
• Genetic Predisposition to Disease
• Genome, Human
• Genome-Wide Association Study
• Gout (genetics)
• Humans
• Hyperuricemia (genetics)
• Kidney Diseases (genetics)
• Kidney Failure, Chronic (genetics)
• Male
• Pedigree
• Uric Acid (blood, metabolism)