[Protective effect of hydroxysafflor yellow A against acute lung injury induced by oleic acid and lipopolysaccharide in rats].

Abstract

This study is to investigate the pharmacological effect and mechanism of action of hydroxysafflor yellow A (HSYA) on acute lung injury (ALI). The rat ALI was induced by oleic acid and lipopolysaccharide (LPS) injection. The incidence of acidosis, PaO2 (arterial blood oxygen pressure), W/D (wet weight/dry weight) and lung index (LI) were measured. Electron microscope and optical microscope were applied to observe lung morphological changes in rat. RT-PCR was used to determine TNF-alpha and ICAM-1 mRNA level. Inhibition effect of HSYA on plasma inflammatory cytokine expression was measured by ELISA. HSYA could alleviate pulmonary edema, reduce acidosis, keep PaO2 from descending, inhibit inflammatory cell infiltration, inhibit rat lung TNF-alpha and ICAM-1 mRNA expression and plasma IL-6 and IL-1beta level elevation. HSYA is an effective ingredient to remit ALI induced by oleic acid and LPS in rat.

• Authors: Xiao-fei Wang, Ming Jin, Jing Tong, Wei Wu, Jin-rong Li, Bao-xia Zang
• Journal: Yao xue xue bao = Acta pharmaceutica Sinica (Yao Xue Xue Bao) Vol. 45 Issue 7 Pg. 940-4 (Jul 2010) ISSN: 0513-4870 [Print] China
• PMID: 20931796 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Interleukin-1beta
• Interleukin-6
• Lipopolysaccharides
• Quinones
• RNA, Messenger
• Tumor Necrosis Factor-alpha
• Intercellular Adhesion Molecule-1
• hydroxysafflor yellow A
• Oleic Acid
• Chalcone

Topics

• Acute Lung Injury (chemically induced, metabolism, pathology)
• Animals
• Carthamus tinctorius (chemistry)
• Chalcone (analogs & derivatives, isolation & purification, pharmacology)
• Flowers (chemistry)
• Intercellular Adhesion Molecule-1 (genetics, metabolism)
• Interleukin-1beta (blood)
• Interleukin-6 (blood)
• Lipopolysaccharides
• Lung (metabolism, pathology, ultrastructure)
• Male
• Oleic Acid
• Plants, Medicinal (chemistry)
• Quinones (isolation & purification, pharmacology)
• RNA, Messenger (metabolism)
• Rats
• Rats, Wistar
• Tumor Necrosis Factor-alpha (genetics, metabolism)