Abstract |
The cell cycle is strictly regulated by numerous mechanisms to ensure cell division. The transcriptional regulation of cell-cycle-related genes is poorly understood, with the exception of the E2F family that governs the cell cycle. Here, we show that a transcription factor, zinc finger protein 143 (ZNF143), positively regulates many cell-cycle-associated genes and is highly expressed in multiple solid tumors. RNA-interference (RNAi)-mediated knockdown of ZNF143 showed that expression of 152 genes was downregulated in human prostate cancer PC3 cells. Among these ZNF143 targets, 41 genes (27%) were associated with cell cycle and DNA replication including cell division cycle 6 homolog (CDC6), polo-like kinase 1 (PLK1) and minichromosome maintenance complex component (MCM) DNA replication proteins. Furthermore, RNAi of ZNF143 induced apoptosis following G2/M cell cycle arrest. Cell growth of 10 lung cancer cell lines was significantly correlated with cellular expression of ZNF143. Our data suggest that ZNF143 might be a master regulator of the cell cycle. Our findings also indicate that ZNF143 is a member of the growing list of non-oncogenes that are promising cancer drug targets.
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Authors | Hiroto Izumi, Tetsuro Wakasugi, Shohei Shimajiri, Akihide Tanimoto, Yasuyuki Sasaguri, Eiji Kashiwagi, Yoshihiro Yasuniwa, Masaki Akiyama, Bin Han, Ying Wu, Takeshi Uchiumi, Tokuzo Arao, Kazuto Nishio, Ryuta Yamazaki, Kimitoshi Kohno |
Journal | Cancer science
(Cancer Sci)
Vol. 101
Issue 12
Pg. 2538-45
(Dec 2010)
ISSN: 1349-7006 [Electronic] England |
PMID | 20860770
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2010 Japanese Cancer Association. |
Chemical References |
- Trans-Activators
- ZNF143 protein, human
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Topics |
- Blotting, Western
- Cell Line, Tumor
- Cell Proliferation
- Cell Separation
- Chromatin Immunoprecipitation
- DNA Replication
(physiology)
- Flow Cytometry
- Gene Expression
- Gene Expression Regulation, Neoplastic
- Genes, cdc
- Humans
- Immunohistochemistry
- Neoplasms
(genetics, metabolism)
- Oligonucleotide Array Sequence Analysis
- RNA Interference
- Trans-Activators
(metabolism)
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