Urinary matrix metalloproteinase-7 level is associated with the presence of metastasis in bladder cancer.

Abstract

OBJECTIVE:

• To assess the presence of matrix metalloproteinase (MMP)-7 in urine samples of patients with bladder cancer and to investigate the correlation between MMP-7 urine concentration and clinicopathological variables.

PATIENTS AND METHODS:

• The presence of MMP-7 in the urine of patients with bladder cancer was tested in 32 representative cases using immunoprecipitation followed by western blot analysis. • Urinary MMP-7 concentration levels were analyzed in 132 patients with bladder cancer and 96 controls using an enzyme-linked immunosorbent assay.

RESULTS:

• MMP-7 levels did not differ significantly between patients with localized bladder cancer and controls (P= 0.174). On the other hand, we detected a fourfold, significantly elevated MMP-7 concentration in urine samples of patients with bladder cancer with regional or distant metastasis (P= 0.003). • Using a threshold value of 6.88 ng/ml, determined by receiver-operating characteristic curve analysis, a specificity of 82% and a sensitivity of 78% were observed. • Western blot analysis revealed that the 55-kDa tissue inhibitor of metalloproteinase 1 complexed MMP-7 is the dominant form of urinary matrilysin.

CONCLUSIONS:

• MMP-7 is present in detectable amounts in the urine of patients with bladder cancer. Its concentrations are significantly elevated in patients with metastatic disease. • Determination of urinary matrilysin level could help to detect bladder cancer metastasis, and may therefore provide a more reliable prognosis and influence therapy decisions.

• Authors: Tibor Szarvas, Bernhard B Singer, Markus Becker, Frank Vom Dorp, Tobias Jäger, Attila Szendroi, Péter Riesz, Imre Romics, Herbert Rübben, Süleyman Ergün
• Journal: BJU international (BJU Int) Vol. 107 Issue 7 Pg. 1069-73 (Apr 2011) ISSN: 1464-410X [Electronic] England
• PMID: 20825402 (Publication Type: Journal Article, Multicenter Study, Research Support, Non-U.S. Gov't)
• Copyright: © 2010 THE AUTHORS. BJU INTERNATIONAL © 2010 BJU INTERNATIONAL.

Chemical References

• Biomarkers, Tumor
• Matrix Metalloproteinase 7

Topics

• Adult
• Aged
• Aged, 80 and over
• Biomarkers, Tumor (blood, urine)
• Blotting, Western
• Enzyme-Linked Immunosorbent Assay
• Female
• Humans
• Male
• Matrix Metalloproteinase 7 (blood, urine)
• Middle Aged
• Prognosis
• Sensitivity and Specificity
• Urinary Bladder Neoplasms (enzymology, pathology, urine)